Probiotic E. coli treatment mediates antimicrobial human beta-defensin synthesis and fecal excretion in humans.
Probiotic E. coli treatment mediates antimicrobial human beta-defensin synthesis and fecal excretion in humans.
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DOI：
10.1038/mi.2008.77
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发表时间：
2009-03
影响因子：
8
通讯作者：
Wehkamp, J.
中科院分区：
文献类型：
--
作者：
Moendel, M.;Schroeder, B. O.;Zimmermann, K.;Huber, H.;Nuding, S.;Beisner, J.;Fellermann, K.;Stange, E. F.;Wehkamp, J.
诱导型人β-防御素（hBD）在肠屏障功能中起重要作用。体外研究表明，临床有效的益生菌诱导抗菌hBD-2。在这里，我们旨在评估健康志愿者的体内效应，并讨论防御素如何影响益生菌的存活。Symbioflor 2含有一个菌株的几个可行的基因型的大肠杆菌给予23名健康人。3周后，粪便hBD-2肽增加78%（平均3.7倍; P<0.0001）。有趣的是，粪便hBD-2肽在治疗后9周仍然升高（P=0.008）。体外研究表明，这种作用仅由三分之一的E。coli基因型，与益生菌E. coli Nissle 1917（10- 15倍）。功能测定表明，所有测试的细菌都被防御素类似地杀死，从而可以推测这种作用的自杀特征。防御素诱导似乎是益生菌治疗的一种常见且重要的机制。
Inducible epithelial human β-defensins (hBD) play an important role in intestinal barrier function. In vitro studies showed that clinically effective probiotics induce antimicrobial hBD-2. Here, we aimed to assess the in vivo effect in healthy volunteers and also addressed how defensins affect probiotic survival. Symbioflor 2 containing one strain of several viable genotypes of Escherichia coli was administered to 23 healthy individuals. After 3 weeks, fecal hBD-2 peptide was increased in 78% (mean 3.7-fold; P<0.0001). Interestingly, the fecal hBD-2 peptide was still elevated 9 weeks after treatment (P=0.008). In vitro studies revealed that this effect was mediated by only one out of three tested E. coli genotypes and comparable to probiotic E. coli Nissle 1917 (10- to 15-fold). Functional assays showed that all tested bacteria were similarly killed by defensins allowing to speculate about a suicidal character of this effect. Defensin induction seems to be a common and important mechanism of probiotic treatment.
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作者：
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