Proteome regulation during Olea europaea fruit development.
Proteome regulation during Olea europaea fruit development.
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OLEA Europaea水果开发期间的蛋白质组调节。
DOI：
10.1371/journal.pone.0053563
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Perrotta G
中科院分区：
文献类型：
--
作者：
Bianco L;Alagna F;Baldoni L;Finnie C;Svensson B;Perrotta G
欧洲油橄榄树广泛分布于地中海盆地，因其从成熟果实中机械提取的油的营养和抗癌特性而受到全世界的欢迎。果实发育是一个生理过程，对影响核果质量的化合物的生物合成以及橄榄油的最终成分的调节有显著影响。蛋白质组学为深入研究果实发育过程中的主要变化提供了可能，包括成熟的重要阶段，并对这些复杂生理过程中发生的蛋白质积累的时间模式进行分类。在这项工作中，我们开始使用比较蛋白质组学耦合质谱法监测与橄榄果实发育相关的蛋白质组变异。从三个不同发育阶段的核果中提取的蛋白质在2-DE上分离并进行图像分析。差异积累蛋白斑247个。从121个位点中鉴定出蛋白质，并讨论了与果实发育过程中核果代谢变化的关系。为了评估在蛋白质水平上观察到的变化是否与mrna的变化一致，本工作中产生的蛋白质组学数据与先前研究中阐述的转录组学数据进行了比较。本研究确定了一些与cv质量性状有关的蛋白质。特别是与脂肪酸、酚类和芳香化合物代谢有关的蛋白质。参与果实光合作用的蛋白质也已被确定，并讨论了它们在油脂形成中的关键作用。迄今为止，该研究首次表征了橄榄果实发育过程中的蛋白质组，为研究果实代谢和油脂积累过程提供了新的见解。
Widespread in the Mediterranean basin, Olea europaea trees are gaining worldwide popularity for the nutritional and cancer-protective properties of the oil, mechanically extracted from ripe fruits. Fruit development is a physiological process with remarkable impact on the modulation of the biosynthesis of compounds affecting the quality of the drupes as well as the final composition of the olive oil. Proteomics offers the possibility to dig deeper into the major changes during fruit development, including the important phase of ripening, and to classify temporal patterns of protein accumulation occurring during these complex physiological processes. In this work, we started monitoring the proteome variations associated with olive fruit development by using comparative proteomics coupled to mass spectrometry. Proteins extracted from drupes at three different developmental stages were separated on 2-DE and subjected to image analysis. 247 protein spots were revealed as differentially accumulated. Proteins were identified from a total of 121 spots and discussed in relation to olive drupe metabolic changes occurring during fruit development. In order to evaluate if changes observed at the protein level were consistent with changes of mRNAs, proteomic data produced in the present work were compared with transcriptomic data elaborated during previous studies. This study identifies a number of proteins responsible for quality traits of cv. Coratina, with particular regard to proteins associated to the metabolism of fatty acids, phenolic and aroma compounds. Proteins involved in fruit photosynthesis have been also identified and their pivotal contribution in oleogenesis has been discussed. To date, this study represents the first characterization of the olive fruit proteome during development, providing new insights into fruit metabolism and oil accumulation process.
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影响因子：
3.3
作者：
Esteve, Clara;D'Amato, Alfonsina;Righetti, Pier Giorgio
通讯作者：
Righetti, Pier Giorgio
影响因子：
6.1
作者：
Dierkes, Georg;Bongartz, Annette;Hayen, Heiko
通讯作者：
Hayen, Heiko
影响因子：
3.8
作者：
AMIOT, MJ;FLEURIET, A;MACHEIX, JJ
通讯作者：
MACHEIX, JJ
影响因子：
6.4
作者：
Etienne, C;Moing, A;Rothan, C
通讯作者：
Rothan, C
DOI：
10.1007/s12094-006-0090-0
发表时间：
2006-01-01
期刊：
Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico
影响因子：
--
作者：
Colomer, Ramon;Menendez, Javier A
通讯作者：
Menendez, Javier A