Differently expressed long noncoding RNAs and mRNAs in TK6 cells exposed to low dose hydroquinone.
Differently expressed long noncoding RNAs and mRNAs in TK6 cells exposed to low dose hydroquinone.
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暴露于低剂量氢醌的 TK6 细胞中不同表达的长非编码 RNA 和 mRNA
DOI：
10.18632/oncotarget.21481
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发表时间：
2017-11-10
期刊：
影响因子：
--
通讯作者：
Tang H
中科院分区：
文献类型：
--
作者：
Chen S;Liang H;Hu G;Yang H;Zhou K;Xu L;Liu J;Lai B;Song L;Luo H;Peng J;Liu Z;Xiao Y;Chen W;Tang H
研究表明，长链非编码RNA（lncRNA）与人类肿瘤的发生有关，可作为诊断和预后的生物标志物。对苯二酚（HQ）是苯的代谢产物之一，与职业性苯中毒和职业性白血病密切相关。采用高通量测序技术，研究了实验组（HQ 20 μ mol/L）和对照组（PBS）的lncRNA和mRNA表达谱的差异。与对照组相比，实验组中总共有65个lncRNA和186个mRNA被鉴定为异常表达超过2倍。为了验证测序结果，我们选择了10个lncRNA和10个mRNA用于定量实时PCR（qRT-PCR）。通过GO注释和KEGG通路分析，我们得到了3条主要的信号通路，其中P53信号通路在肿瘤的发生发展中起着重要的作用。之后，构建了25个lncRNA和32个mRNAs组成的lncRNA-mRNA共表达网络，通过调控基因表达发挥失调的lncRNA转录本的生物学功能。lncRNA靶基因预测为lncRNA的研究提供了新的思路。最后，我们还有一个重要的发现，筛选出11个新的lncRNA，没有进行注释。这些结果揭示了低剂量HQ作用后TK 6细胞lncRNA和mRNA表达谱与对照组不同，有助于进一步研究HQ的毒性机制，为白血病的治疗提供了新的方向。
Previous studies have shown that long noncoding RNAs (lncRNAs) were related to human carcinogenesis and might be designated as diagnosis and prognosis biomarkers. Hydroquinone (HQ), as one of the metabolites of benzene, was closely relevant to occupational benzene poisoning and occupational leukemia. Using high-throughput sequencing technology, we investigated differences in lncRNA and mRNA expression profiles between experimental group (HQ 20 μmol/L) and control group (PBS). Compared to control group, a total of 65 lncRNAs and 186 mRNAs were previously identified to be aberrantly expressed more than two fold change in experimental group. To validate the sequencing results, we selected 10 lncRNAs and 10 mRNAs for quantitative real-time PCR (qRT-PCR). Through GO annotation and KEGG pathway analysis, we obtained 3 mainly signaling pathways, including P53 signaling pathway, which plays an important role in tumorigenesis and progression. After that, 25 lncRNAs and 32 mRNAs formed the lncRNA-mRNA co-expression network were implemented to play biological functions of the dysregulated lncRNAs transcripts by regulating gene expression. The lncRNAs target genes prediction provided a new idea for the study of lncRNAs. Finally, we have another important discovery, which is screened out 11 new lncRNAs without annotated. All these results uncovered that lncRNA and mRNA expression profiles in TK6 cells exposed to low dose HQ were different from control group, helping to further study the toxicity mechanisms of HQ and providing a new direction for the therapy of leukemia.
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影响因子：
1
作者：
Ugrin, Milena;Stojiljkovic, Maja;Pavlovic, Sonja
通讯作者：
Pavlovic, Sonja
影响因子：
14.9
作者：
Kanehisa M;Goto S;Sato Y;Furumichi M;Tanabe M
通讯作者：
Tanabe M
DOI：
10.1093/bioinformatics/btr209
发表时间：
2011-07-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Lin MF;Jungreis I;Kellis M
通讯作者：
Kellis M
影响因子：
3.4
作者：
Li J;Xu Y;Xu J;Wang J;Wu L
通讯作者：
Wu L
影响因子：
11.4
作者：
Blume, C. J.;Hotz-Wagenblatt, A.;Zenz, T.
通讯作者：
Zenz, T.