Clinical cell therapy imaging using a perfluorocarbon tracer and fluorine-19 MRI.
Clinical cell therapy imaging using a perfluorocarbon tracer and fluorine-19 MRI.
复制标题
DOI：
10.1002/mrm.25454
复制
发表时间：
2014-12
影响因子：
3.3
通讯作者：
Schirda, Claudiu
中科院分区：
文献类型：
--
作者：
Ahrens, Eric T.;Helfer, Brooke M.;O'Hanlon, Charles F.;Schirda, Claudiu
细胞疗法正在成为许多严重人类疾病的一种治疗选择。为了加速临床转化，临床试验中细胞移植物的无创成像可以潜在地用于评估细胞的初始传递和行为。使用全氟化碳（PFC）示踪剂用于临床氟-19 (19F) MRI细胞检测。该技术被用于检测免疫治疗树突状细胞（dc）递送给结直肠癌患者。体外用PFC MRI试剂标记自身DC疫苗。患者接受皮内dc，采用3tesla (T) 19F自旋密度加权MRI观察细胞。注射部位的自旋密度加权19F图像显示dc为无背景的“热点”图像。在临床相关扫描时间（<10 min）获得19F图像。在两名患者中，从19F热点可以量化表观DC数，并观察到注射部位在24小时内减少了~ 50%。从3T幻影研究中，DC检测的灵敏度极限估计在本研究中约为~ 105个细胞/体素。这些结果有助于建立一种临床适用的方法来跟踪细胞治疗中使用的广泛的细胞类型。中国生物医学工程杂志（英文版），2014。©2014作者。医学磁共振由Wiley期刊公司代表国际共振医学学会出版。
Cellular therapeutics are emerging as a treatment option for a host of serious human diseases. To accelerate clinical translation, noninvasive imaging of cell grafts in clinical trials can potentially be used to assess the initial delivery and behavior of cells. The use of a perfluorocarbon (PFC) tracer agent for clinical fluorine-19 (19F) MRI cell detection is described. This technology was used to detect immunotherapeutic dendritic cells (DCs) delivered to colorectal adenocarcinoma patients. Autologous DC vaccines were labeled with a PFC MRI agent ex vivo. Patients received DCs intradermally, and 19F spin-density-weighted MRI at 3 Tesla (T) was used to observe cells. Spin-density-weighted 19F images at the injection site displayed DCs as background-free “hot-spot” images. 19F images were acquired in clinically relevant scan times (<10 min). Apparent DC numbers could be quantified in two patients from the 19F hot-spots and were observed to decrease by ∼50% at injection site by 24 h. From 3T phantom studies, the sensitivity limit for DC detection is estimated to be on the order of ∼105 cells/voxel in this study. These results help to establish a clinically applicable means to track a broad range of cell types used in cell therapy. Magn Reson Med 72:1696–1701, 2014. © 2014 The Authors. Magnetic Resonance in Medicine Published by Wiley Periodicals, Inc. on behalf of International Society of Medicine in Resonance.
登录
查看更多内容
影响因子：
3.3
作者：
Srinivas, Mangala;Turner, Michael S.;Janjic, Jelena M.;Morel, Penelope A.;Laidlaw, David H.;Ahrens, Eric T.
通讯作者：
Ahrens, Eric T.
影响因子：
46.9
作者：
Ahrens, ET;Flores, R;Morel, PA
通讯作者：
Morel, PA
带有嵌合抗原受体的T细胞具有有效的抗肿瘤作用，可以在晚期白血病患者中建立记忆。
影响因子：
17.1
作者：
Kalos M;Levine BL;Porter DL;Katz S;Grupp SA;Bagg A;June CH
通讯作者：
June CH
影响因子：
4.5
作者：
Helfer BM;Balducci A;Nelson AD;Janjic JM;Gil RR;Kalinski P;de Vries IJ;Ahrens ET;Mailliard RB
通讯作者：
Mailliard RB
影响因子：
3.3
作者：
Srinivas, Mangala;Morel, Penelope A.;Ahrens, Eric T.
通讯作者：
Ahrens, Eric T.