Functional assessment of human dendritic cells labeled for in vivo (19)F magnetic resonance imaging cell tracking.
Functional assessment of human dendritic cells labeled for in vivo (19)F magnetic resonance imaging cell tracking.
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DOI：
10.3109/14653240903446902
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发表时间：
2010-04
期刊：
影响因子：
4.5
通讯作者：
Mailliard RB
中科院分区：
文献类型：
--
作者：
Helfer BM;Balducci A;Nelson AD;Janjic JM;Gil RR;Kalinski P;de Vries IJ;Ahrens ET;Mailliard RB
树突状细胞（DC）越来越多地被用作治疗癌症和感染性疾病的细胞疫苗。虽然在使用基于DC的疫苗的早期临床试验中已经有一些有希望的结果，但是一旦过继转移到患者体内，无法非侵入性地可视化细胞的位置、迁移和命运通常被认为是这些疗法进步的限制因素。一种新型的全氟聚醚（PFPE）示踪剂被用来标记人DC离体的目的是跟踪细胞在体内通过19 F磁共振成像（MRI）。我们对这项技术进行了评估，并研究了其对DC健康和功能的影响。用PFPE标记单核细胞来源的DC，然后进行评估。通过检查细胞膜完整性和线粒体脂质含量测定细胞活力。免疫组化和流式细胞术检测DC成熟标志物的表面抗原表达。功能测试包括白细胞介素（IL）-12p70生产，T细胞刺激功能和趋化性的生物测定。通过将PFPE标记的人DC接种到NOD-SCID小鼠中来证明MRI功效。用PFPE有效地标记DC，而对细胞活力、表型或功能没有显著影响。PFPE标记的DC在体内通过19 F MRI清楚地检测到，成熟DC显示在18 h内选择性地向引流淋巴结区域迁移。这项研究是PFPE细胞标记和MRI细胞跟踪使用人类免疫细胞的首次应用。这些技术在未来的临床试验中可能具有跟踪治疗细胞的巨大潜力。
Dendritic cells (DC) are increasingly being used as cellular vaccines to treat cancer and infectious diseases. While there have been some promising results in early clinical trials using DC-based vaccines, the inability to visualize non-invasively the location, migration and fate of cells once adoptively transferred into patients is often cited as a limiting factor in the advancement of these therapies. A novel perflouropolyether (PFPE) tracer agent was used to label human DC ex vivo for the purpose of tracking the cells in vivo by 19F magnetic resonance imaging (MRI). We provide an assessment of this technology and examine its impact on the health and function of the DC. Monocyte-derived DC were labeled with PFPE and then assessed. Cell viability was determined by examining cell membrane integrity and mitochondrial lipid content. Immunostaining and flow cytometry were used to measure surface antigen expression of DC maturation markers. Functional tests included bioassays for interleukin (IL)-12p70 production, T-cell stimulatory function and chemotaxis. MRI efficacy was demonstrated by inoculation of PFPE-labeled human DC into NOD-SCID mice. DC were effectively labeled with PFPE without significant impact on cell viability, phenotype or function. The PFPE-labeled DC were clearly detected in vivo by 19F MRI, with mature DC being shown to migrate selectively towards draining lymph node regions within 18 h. This study is the first application of PFPE cell labeling and MRI cell tracking using human immunotherapeutic cells. These techniques may have significant potential for tracking therapeutic cells in future clinical trials.
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影响因子：
82.9
作者：
Figdor, CG;de Vries, IJM;Melief, CJM
通讯作者：
Melief, CJM
影响因子：
46.9
作者：
Ahrens, ET;Flores, R;Morel, PA
通讯作者：
Morel, PA
影响因子：
30.5
作者：
Langenkamp, A;Messi, M;Sallusto, F
通讯作者：
Sallusto, F
影响因子：
15.3
作者：
Dieu, M C;Vanbervliet, B;Vicari, A;Bridon, J M;Oldham, E;Ait-Yahia, S;Briere, F;Zlotnik, A;Lebecque, S;Caux, C
通讯作者：
Caux, C
影响因子：
15
作者：
Janjic, Jelena M.;Srinivas, Mangala;Ahrens, Eric T.
通讯作者：
Ahrens, Eric T.