Dynamic behavior of Salmonella-induced membrane tubules in epithelial cells.
Dynamic behavior of Salmonella-induced membrane tubules in epithelial cells.
复制标题
沙门氏菌诱导的上皮细胞膜管的动态行为。
DOI：
10.1111/j.1600-0854.2008.00830.x
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发表时间：
2008-12
期刊：
影响因子：
--
通讯作者：
Steele-Mortimer O
中科院分区：
文献类型：
--
作者：
Drecktrah D;Levine-Wilkinson S;Dam T;Winfree S;Knodler LA;Schroer TA;Steele-Mortimer O
鼠伤寒沙门氏菌是一种兼性细胞内病原体，可引起急性肠胃炎。胞内沙门氏菌在被称为含沙门氏菌液泡（SCV）的经过修饰的吞噬体中存活和复制。细胞内复制的开始伴随着膜小管的出现，称为沙门氏菌诱导的细丝（Sifs），从SCV延伸。Sifs富含溶酶体晚期膜蛋白，如溶酶体相关膜蛋白1，但其形成和与内体室相互作用的能力尚不清楚。在这项研究中，我们使用活细胞成像技术来定义感染上皮细胞中Sif形成的动力学。在早期时间点，Sifs是从scv表面延伸出来的简单小管。这些小管是高度动态的，表现出双向的、微管依赖的运动。在离SCV最远的单个Sif小管的远端，经常观察到一个明显的“先导”结构域。在后期，Sifs发育成高度复杂的管状网络，延伸到整个细胞，比新生的Sifs显得不那么动态；然而，单个小管继续表现出双向动力学。Sifs可以通过融合获得内吞内容，表明与内吞途径持续相互作用。总之，这些结果表明，这些沙门氏菌诱导的小管形成了一个高度动态的网络，包括微管依赖的运动和与内体隔室的相互作用。
Salmonella Typhimurium is a facultative intracellular pathogen that causes acute gastroenteritis in man. Intracellular Salmonella survive and replicate within a modified phagosome known as the Salmonella-containing vacuole (SCV). The onset of intracellular replication is accompanied by the appearance of membrane tubules, called Salmonella-induced filaments (Sifs), extending from the SCV. Sifs are enriched in late endosomal/lysosomal membrane proteins such as lysosome-associated membrane protein 1, but their formation and ability to interact with endosomal compartments are not characterized. In this study, we use live cell imaging techniques to define the dynamics of Sif formation in infected epithelial cells. At early time-points, Sifs are simple tubules extending from the surface of SCVs. These tubules are highly dynamic and exhibit bidirectional, microtubule-dependent movement. At the distal ends of individual Sif tubules, furthest from the SCV, a distinct ‘leader’ domain was often observed. At later times, Sifs develop into highly complex tubular networks that extend throughout the cell and appear less dynamic than nascent Sifs; however, individual tubules continue to display bidirectional dynamics. Sifs can acquire endocytic content by fusion, indicating a sustained interaction with the endocytic pathway. Together, these results show that these Salmonella-induced tubules form a highly dynamic network that involves both microtubule-dependent motility and interactions with endosomal compartments.
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将鼠伤寒沙门氏菌靶向含有溶酶体膜糖蛋白的囊泡，绕过带有甘露糖6-磷酸受体的室。
影响因子：
7.8
作者：
GARCIADELPORTILLO, F;FINLAY, BB
通讯作者：
FINLAY, BB
影响因子：
4.8
作者：
Boucrot, E;Beuzón, CR;Méresse, S
通讯作者：
Méresse, S
DOI：
10.1083/jcb.137.1.113
发表时间：
1997-04-07
期刊：
The Journal of cell biology
影响因子：
--
作者：
Blocker A;Severin FF;Burkhardt JK;Bingham JB;Yu H;Olivo JC;Schroer TA;Hyman AA;Griffiths G
通讯作者：
Griffiths G
影响因子：
3.3
作者：
Ko, DC;Gordon, MD;Scott, MP
通讯作者：
Scott, MP
影响因子：
56.9
作者：
Boucrot, E;Henry, T;Méresse, S
通讯作者：
Méresse, S