A novel betaine aldehyde dehydrogenase gene from Jatropha curcas, encoding an enzyme implicated in adaptation to environmental stress
A novel betaine aldehyde dehydrogenase gene from Jatropha curcas, encoding an enzyme implicated in adaptation to environmental stress
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来自麻风树的一种新型甜菜碱醛脱氢酶基因，编码一种与适应环境胁迫有关的酶
DOI：
10.1016/j.plantsci.2008.01.018
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发表时间：
2008-05
期刊：
影响因子：
5.2
通讯作者：
Niu, Bei
中科院分区：
文献类型：
--
作者：
Tang, Lin;Wu, Jun;Zhang, Fu-Li;Chen, Fang;Xu, Ying;Jiang, Lu-Ding;Jia, Yong-Rong;Wang, Ying-Chun;Wang, Sheng-Hua;Gao, Shun;Niu, Bei
利用RT-PCR和RACE技术从麻风树中克隆到一个甜菜碱醛脱氢酶基因，命名为JcBD 1，一种重要的植物物种，其种子是生物柴油的原料。该基因全长1769 bp，开放阅读框为1509 bp，编码503个氨基酸，分子量为54.585 kD，等电点为5.32。推导的氨基酸序列与其他植物中存在的BADH酶有很大的相似性，包括可能的活性位点的绝对保守区域。Southern杂交分析表明，JcBD 1基因属于一个多基因家族。半定量RT-PCR分析表明，JcBD 1基因在根、茎、叶、花和幼嫩种子中均有表达，但在叶和茎中表达量最高。JcBD 1基因在干旱（30%PEG）、高温（50°C）和盐（300 mM NaCl）等环境胁迫下的叶片中表达增加。JcBD 1蛋白在大肠杆菌（Escherichia coli，E.大肠杆菌）。E.转化JcBD 1基因的大肠杆菌细胞生长明显优于对照细胞。检测表达的JcBD 1蛋白的酶活性。JcBD 1酶在E.大肠杆菌赋予其对盐等非生物胁迫的抗性。
A novel betaine aldehyde dehydrogenase gene, named JcBD1, was cloned by RT-PCR and RACE techniques from Jatropha curcas L., an important plant species as its seed is the raw material for biodiesels. The cDNA was 1769bp in length with a complete open reading frame of 1509bp, encoding 503 amino acids with a predicted molecular mass of 54.585kD and a pI of 5.32. The deduced amino acid sequence showed substantial similarities to BADH enzymes present in other plants, including the absolute conservative region in the possible active site. Southern blot analysis indicated that the JcBD1 gene belongs to a multigene family. Semi-quantitative RT-PCR analyses revealed that JcBD1 gene was expressed in roots, stems, leaves, flowers, and young seeds, but that the expression level was highest in leaves and stems. And the JcBD1 gene was increasedly expressed in leaves undergoing environmental stress like drought (30% PEG), heat (50°C), and salt (300mM NaCl). The JcBD1 protein was functionally expressed in Escherichia coli (E. coli). E. coli cells transformed with the JcBD1 gene obviously grew better than control cells. The enzyme activity of expressed JcBD1 protein was detected. Over-expression of JcBD1 enzyme in E. coli conferred its resistance to abiotic stressors like salt.
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影响因子：
2.7
作者：
Qiuli Li;Xiao-rong Gao;Xiaohong Yu;Xingzhi Wang;L. An
通讯作者：
Qiuli Li;Xiao-rong Gao;Xiaohong Yu;Xingzhi Wang;L. An
影响因子：
6.1
作者：
A. Wood;H. Saneoka;D. Rhodes;R. Joly;P. Goldsbrough
通讯作者：
A. Wood;H. Saneoka;D. Rhodes;R. Joly;P. Goldsbrough
影响因子：
4.3
作者：
G. S. Premachandra;D. Hahn;R. Joly
通讯作者：
G. S. Premachandra;D. Hahn;R. Joly
影响因子：
7.3
作者：
Chen, WP;Li, PH;Chen, THH
通讯作者：
Chen, THH
影响因子：
7.4
作者：
Reumann, S
通讯作者：
Reumann, S