The irritant receptor TRPA1 mediates the mosquito repellent effect of catnip.
The irritant receptor TRPA1 mediates the mosquito repellent effect of catnip.
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DOI：
10.1016/j.cub.2021.02.010
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发表时间：
2021-05-10
期刊：
影响因子：
--
通讯作者：
Stensmyr MC
中科院分区：
文献类型：
--
作者：
Melo N;Capek M;Arenas OM;Afify A;Yilmaz A;Potter CJ;Laminette PJ;Para A;Gallio M;Stensmyr MC
猫薄荷（荆芥cataria）是一种常见的花园草药，以其对家猫的欣快和致幻作用，其药用特性以及对昆虫的强大驱避作用而闻名。猫薄荷提取物已被提议作为合成驱虫剂（如N，N-二乙基-3-甲基苯甲酰胺（DEET））的天然替代品，但猫薄荷如何引发昆虫的厌恶尚不清楚。在这里，我们表明，无论是在果蝇和埃及伊蚊，猫薄荷驱避的主要介质是广泛保守的化学刺激受体TRPA 1。在体外，猫薄荷提取物及其活性成分荆芥内酯都可以直接激活苍蝇和蚊子的TRPA 1。在体内，D. melanogaster和Ae.埃及TRPA 1突变体不再受到猫薄荷和荆芥内酯的排斥。有趣的是，我们的数据显示，一些，但不是全部，苍蝇和蚊子TRPA 1变体是猫薄荷的目标。此外，与广泛的TRPA 1激动剂异硫氰酸烯丙酯（AITC）（催泪瓦斯和芥末的活性成分）不同，猫薄荷不会激活人类TRPA 1。我们的研究结果支持猫薄荷和荆芥内酯作为昆虫选择性刺激物的使用，并表明，尽管TRPA 1的广泛保护，昆虫TRPA 1可以有针对性的安全驱虫剂的发展。猫薄荷作为驱虫剂已经使用了几千年。梅洛等人发现猫薄荷及其主要的环烯醚萜成分荆芥内酯激活刺激性受体TRPA 1的昆虫同种型。缺乏TRPA 1的蚊子不再被猫薄荷排斥。猫薄荷不会激活人类TRPA 1，这支持其作为安全的天然驱蚊剂的用途。
Catnip (Nepeta cataria) is a common garden herb well known for its euphoric and hallucinogenic effects on domestic cats, for its medicinal properties, as well as for its powerful repellent action on insects. Catnip extracts have been proposed as a natural alternative to synthetic insect repellents, such as N,N-diethyl-3-methylbenzamide (DEET), but how catnip triggers aversion in insects is not known. Here, we show that, both in Drosophila melanogaster flies and Aedes aegypti mosquitoes, the major mediator of catnip repellency is the widely conserved chemical irritant receptor TRPA1. In vitro, both catnip extract and its active ingredient nepetalactone can directly activate fly and mosquito TRPA1. In vivo, D. melanogaster and Ae. aegypti TRPA1 mutants are no longer repelled by catnip and nepetalactone. Interestingly, our data show that some, but not all, fly and mosquito TRPA1 variants are catnip targets. Moreover, unlike the broad TRPA1 agonist allyl isothiocyanate (AITC) (an active ingredient of tear gas and wasabi), catnip does not activate human TRPA1. Our results support the use of catnip and nepetalactone as insect-selective irritants and suggest that, despite TRPA1’s broad conservation, insect TRPA1 can be targeted for the development of safe repellents. Catnip has been used for millennia as an insect repellent. Melo et al. find that catnip and its major iridoid component nepetalactone activate insect isoforms of the irritant receptor TRPA1. Mosquitoes lacking TRPA1 are no longer repelled by catnip. Catnip does not activate human TRPA1, and this supports its use as a safe natural mosquito repellent.
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影响因子：
4.4
作者：
Lombardo F;Salvemini M;Fiorillo C;Nolan T;Zwiebel LJ;Ribeiro JM;Arcà B
通讯作者：
Arcà B
影响因子：
2.6
作者：
Bol S;Caspers J;Buckingham L;Anderson-Shelton GD;Ridgway C;Buffington CA;Schulz S;Bunnik EM
通讯作者：
Bunnik EM
影响因子：
64.8
作者：
通讯作者：
--
DOI：
10.1523/jneurosci.4237-12.2013
发表时间：
2013-04-17
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Lee Y;Montell C
通讯作者：
Montell C
影响因子：
3.8
作者：
Birkett, Michael A.;Hassanali, Ahmed;Pickett, John A.
通讯作者：
Pickett, John A.