Deciphering the olfactory repertoire of the tiger mosquito Aedes albopictus.
Deciphering the olfactory repertoire of the tiger mosquito Aedes albopictus.
复制标题
DOI：
10.1186/s12864-017-4144-1
复制
发表时间：
2017-10-11
期刊：
影响因子：
4.4
通讯作者：
Arcà B
中科院分区：
文献类型：
--
作者：
Lombardo F;Salvemini M;Fiorillo C;Nolan T;Zwiebel LJ;Ribeiro JM;Arcà B
亚洲虎蚊白纹伊蚊（Aedes albopictus）是一种高度侵入性物种，是几种具有公共卫生重要性的虫媒病毒（例如登革热、基孔肯雅热、寨卡病毒）和寄生虫（例如恶丝虫）的有效载体。与其他蚊子相比，Ae。雌性白纹伊蚊表现出一种多面手的寄主寻找以及一种非常具有攻击性的叮咬行为，这是造成其高度滋扰的原因。一些复杂的蚊子行为，如寻找宿主，进食，交配或产卵依赖于嗅觉刺激，这些刺激针对主要位于专门的头部附属物如触角，下颚须和口器上的一系列感觉神经元。目的是描述Ae。我们已经使用RNA-seq来揭示雌性触角和下颚须的转录组谱。采用雄性头部和整个雌性身体作为差异表达分析的参考。评估了每个组织内的相对转录本丰度（TPM，每百万千碱基的转录本）和不同组织中的成对差异丰度（倍数变化值和错误发现率）。鉴定了触角（620）和下颚须（268）中上调的重叠群，并分析了相对GO和PFAM富集谱。化学感受基因被描述为：通过比较基因组学和转录组学共鉴定出77种气味结合蛋白（OBP）、82种气味受体（OR）、60种离子型受体（IR）和30种味觉受体（GR）。此外，直系同源基因表达的女性/男性上颌骨的触须和/或天线，并参与温度感觉（如发热和arrestin1），机械感觉（如压电和无痛）和神经调节进行了分类。我们在这里提供了主Ae的第一个详细的转录组。白纹伊蚊的感觉附属物，即触角和下颚须。对虎蚊嗅觉系统的深入了解将有助于更好地了解其生物学，并可能为设计新的引诱剂/驱避剂铺平道路。本文的在线版本（10.1186/s12864 - 017 - 4144 - 1）包含补充材料，可供授权用户使用。
The Asian tiger mosquito Aedes albopictus is a highly invasive species and competent vector of several arboviruses (e.g. dengue, chikungunya, Zika) and parasites (e.g. dirofilaria) of public health importance. Compared to other mosquito species, Ae. albopictus females exhibit a generalist host seeking as well as a very aggressive biting behaviour that are responsible for its high degree of nuisance. Several complex mosquito behaviours such as host seeking, feeding, mating or oviposition rely on olfactory stimuli that target a range of sensory neurons localized mainly on specialized head appendages such as antennae, maxillary palps and the mouthparts. With the aim to describe the Ae. albopictus olfactory repertoire we have used RNA-seq to reveal the transcriptome profiles of female antennae and maxillary palps. Male heads and whole female bodies were employed as reference for differential expression analysis. The relative transcript abundance within each tissue (TPM, transcripts per kilobase per million) and the pairwise differential abundance in the different tissues (fold change values and false discovery rates) were evaluated. Contigs upregulated in the antennae (620) and maxillary palps (268) were identified and relative GO and PFAM enrichment profiles analysed. Chemosensory genes were described: overall, 77 odorant binding proteins (OBP), 82 odorant receptors (OR), 60 ionotropic receptors (IR) and 30 gustatory receptors (GR) were identified by comparative genomics and transcriptomics. In addition, orthologs of genes expressed in the female/male maxillary palps and/or antennae and involved in thermosensation (e.g. pyrexia and arrestin1), mechanosensation (e.g. piezo and painless) and neuromodulation were classified. We provide here the first detailed transcriptome of the main Ae. albopictus sensory appendages, i.e. antennae and maxillary palps. A deeper knowledge of the olfactory repertoire of the tiger mosquito will help to better understand its biology and may pave the way to design new attractants/repellents. The online version of this article (10.1186/s12864-017-4144-1) contains supplementary material, which is available to authorized users.
登录
查看更多内容
影响因子：
5.7
作者：
Freeman EG;Dahanukar A
通讯作者：
Dahanukar A
影响因子：
2.1
作者：
Armbruster, Peter;White, Sheryl;Zhao, Xiumei
通讯作者：
Zhao, Xiumei
影响因子：
2.6
作者：
Bohbot, J.;Pitts, R. J.;Zwiebel, L. J.
通讯作者：
Zwiebel, L. J.
影响因子：
7.7
作者：
Corfas, Roman A.;Vosshall, Leslie B.
通讯作者：
Vosshall, Leslie B.
影响因子：
9.6
作者：
Bonizzoni M;Gasperi G;Chen X;James AA
通讯作者：
James AA