B-1 cells in the bone marrow are a significant source of natural IgM.
B-1 cells in the bone marrow are a significant source of natural IgM.
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DOI：
10.1002/eji.201141890
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发表时间：
2012-01
影响因子：
5.4
通讯作者：
Baumgarth, Nicole
中科院分区：
文献类型：
--
作者：
Choi, Youn Soo;Dieter, Jacquelyn A.;Rothaeusler, Kristina;Luo, Zheng;Baumgarth, Nicole
在没有抗原挑战的情况下分泌的天然IgM抗体是抗微生物免疫和组织稳态的重要贡献者。早期的研究已经确定骨髓和脾脏（在较小程度上）是这种自发分泌IgM的主要组织来源。然而，负责的B细胞亚群从未被确定。利用多色流式细胞术、细胞分选和嵌合小鼠（其中B-1和B-2细胞及其分泌的抗体由其igg异型区分），我们明确地在脾脏中鉴定出天然的IgM分泌细胞，并首次在骨髓中鉴定出IgM+ IgDlo/−CD19hi CD43+ CD5+/−B-1细胞。新发现的骨髓B-1细胞群显示出脾B-1细胞的许多表型特征，但与腹腔B-1细胞不同，腹腔B-1细胞最多只能产生非常少量的IgM。分泌抗体的脾和骨髓B-1细胞也不同于由抗原诱导的常规B细胞产生的终末分化浆细胞，因为它们表达高水平的表面IgM和CD19，而缺乏CD138的表达。总之，该研究确定了脾脏和骨髓中非终末分化的B-1细胞群体是天然IgM的最重要生产者。
Natural IgM antibodies secreted in the absence of antigenic challenge are important contributors to antimicrobial immunity and tissue homeostasis. Early studies had identified bone marrow and, to a lesser extent the spleen, as main tissue sources of this spontaneously secreted IgM. However, the responsible B cell subset has never been identified. Using multicolor flow cytometry, cell sorting and chimeric mice in which B-1 and B-2 cells and their secreted antibodies are distinguished by their Ig-allotype, we unequivocally identify the natural IgM secreting cells in spleen, and for the first time in the bone marrow as IgM+ IgDlo/−CD19hi CD43+ CD5+/− B-1 cells. The newly identified population of bone marrow B-1 cells shows many of the phenotypic characteristics of splenic B-1 cells but is distinct from B-1 cells in the peritoneal cavity, which generate at best very small amounts of IgM. Antibody-secreting spleen and bone marrow B-1 cells are distinct also from terminally differentiated plasma cells generated from antigen-induced conventional B cells, as they express high levels of surface IgM and CD19 and lack expression of CD138. Together the study identifies populations of non-terminally differentiated B-1 cells in spleen and bone marrow as the most significant producers of natural IgM.
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影响因子：
32.4
作者：
Kallies, Axel;Hasbold, Jhagvaral;Nutt, Stephen L.
通讯作者：
Nutt, Stephen L.
影响因子：
4.4
作者：
Fairfax, Kirsten A.;Corcoran, Lynn M.;Tarlinton, David M.
通讯作者：
Tarlinton, David M.
影响因子：
5.4
作者：
Jayasekera, Jerome P.;Moseman, E. Ashley;Carroll, Michael C.
通讯作者：
Carroll, Michael C.
通过信号通过Toll样受体来调节B1细胞迁移。
DOI：
10.1084/jem.20061041
发表时间：
2006-10-30
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Ha SA;Tsuji M;Suzuki K;Meek B;Yasuda N;Kaisho T;Fagarasan S
通讯作者：
Fagarasan S
DOI：
10.1126/science.1168988
发表时间：
2009-03-27
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Chen GY;Tang J;Zheng P;Liu Y
通讯作者：
Liu Y