3D reconstructions of quail-chick chimeras provide a new fate map of the avian scapula.

3D reconstructions of quail-chick chimeras provide a new fate map of the avian scapula.
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鹌鹑雏鸡嵌合体的 3D 重建提供了鸟类肩胛骨的新命运图。

DOI:
10.1016/j.ydbio.2011.03.032
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发表时间:
2011
影响因子:
2.7
通讯作者:
Burke,AnnC
Burke,AnnC
中科院分区:
生物学3区
文献类型:
--
作者:
Shearman,RebeccaM;Tulenko,FrankJ;Burke,AnnC

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有肢脊椎动物具有功能整合的颅后轴和附肢系统,这些系统源自两个不同的胚胎中胚层群体。轴向骨骼元件源自近轴体节,附肢骨骼和胸骨源自体细胞侧板中胚层,并且两个系统的所有肌肉均源自体节。最近对羊膜动物的研究表明,肩胛骨具有混合的中胚层起源。在这里,我们确定了鹌鹑雏鸡嵌合体体节和侧板中胚层对鸟类肩胛骨的相对贡献。我们对宿主体内的移植组织进行了 3D 重建,揭示了肩胛骨中体细胞的分布与之前报道的截然不同。这种体节和侧板群体之间神秘边界的新颖 3D 可视化揭示了肌肉骨骼形态发生的动态,并证明了嵌合体数据 3D 可视化的重要性。嵌合体的重建清楚地表明了与现有肩胛发育模型的三个显着对比。首先,鸟类肩胛骨的大部分是侧板衍生的,并且对肩胛骨的体节贡献明显小于以前的模型。其次,叶片体节成分的分割部分丢失;第三,来自同一体节的不同组织之间的生长速度存在显着差异,这些组织有助于颈胸过渡段的结构,包括肩胛骨。这些数据要求重新评估有关脊椎动物肩胛骨的发育、同源性和进化的理论。
Limbed vertebrates have functionally integrated postcranial axial and appendicular systems derived from two distinct populations of embryonic mesoderm. The axial skeletal elements arise from the paraxial somites, the appendicular skeleton and sternum arise from the somatic lateral plate mesoderm, and all of the muscles for both systems arise from the somites. Recent studies in amniotes demonstrate that the scapula has a mixed mesodermal origin. Here we determine the relative contribution of somitic and lateral plate mesoderm to the avian scapula from quail-chick chimeras. We generate 3D reconstructions of the grafted tissue in the host revealing a very different distribution of somitic cells in the scapula than previously reported. This novel 3D visualization of the cryptic border between somitic and lateral plate populations reveals the dynamics of musculoskeletal morphogenesis and demonstrates the importance of 3D visualization of chimera data. Reconstructions of chimeras make clear three significant contrasts with existing models of scapular development. First, the majority of the avian scapula is lateral plate derived and the somitic contribution to the scapular blade is significantly smaller than in previous models. Second, the segmentation of the somitic component of the blade is partially lost; and third, there are striking differences in growth rates between different tissues derived from the same somites that contribute to the structures of the cervical thoracic transition, including the scapula. These data call for the reassessment of theories on the development, homology, and evolution of the vertebrate scapula.
禽类胚胎翅膀肌肉组织起源的实验分析
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