In-Plane, In-Series Nanopores with Circular Cross Sections for Resistive-Pulse Sensing.
In-Plane, In-Series Nanopores with Circular Cross Sections for Resistive-Pulse Sensing.
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DOI：
10.1021/acsnano.1c08680
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发表时间：
2022-05-24
期刊：
影响因子：
17.1
通讯作者：
Jacobson, Stephen C.
中科院分区：
文献类型：
--
作者：
Zhang, Mi;Harms, Zachary D.;Greibe, Tine;Starr, Caleb A.;Zlotnick, Adam;Jacobson, Stephen C.
关键词：
利用固态纳米孔的电阻脉冲传感是一种用于分析溶液中单个分子的灵敏、无标记技术。为了向连续脉冲测量添加功能，将纳米孔直接耦合到其他孔和纳米级流体元件，例如，反应器、分离器和过滤器在同一设备中的应用是重要的下一步。一种方法是在基底的平面中整体制造流体元件，但是需要产生具有圆形横截面的孔的方法来改善平面内装置的感测性能。在这里，我们报告了一种制造方法，直接图案的纳米孔与圆形横截面的系列和平面与基板。使用聚焦离子束（FIB）仪器研磨纳米通道中的薄片，随后通过薄片钻一个纳米孔。纳米孔的直径和几何形状由离子束的电流和剂量以及薄层的倾斜角和厚度控制。我们制作了垂直和倾斜的薄片和直径从40到90纳米的圆柱形和圆锥形几何形状的纳米孔的设备。为了测试设备的性能，我们对B型肝炎病毒（HBV）衣壳进行了连续脉冲测量。来自T = 3衣壳（~31 nm直径）和T = 4衣壳（~35 nm直径）的电流脉冲被很好地分辨，并且表现出比在具有矩形横截面的纳米孔上获得的数据高5倍的相对脉冲幅度（Δi/i）。对于接近衣壳直径的较小孔径（≤ 45 nm），对于T = 3和T = 4衣壳均观察到脉冲幅度的显著增加。串联制造的两个和三个孔进一步提高了T = 3和T = 4衣壳的相对脉冲幅度分布之间的分辨率高达2倍。
Resistive-pulse sensing with solid-state nanopores is a sensitive, label-free technique for analyzing single molecules in solution. To add functionality to resistive-pulse measurements, direct coupling of the nanopores to other pores and nanoscale fluidic elements, e.g., reactors, separators, and filters, in the same device is an important next step. One approach is monolithic fabrication of the fluidic elements in the plane of the substrate, but methods to generate pores with circular cross sections are needed to improve sensing performance with in-plane devices. Here, we report a fabrication method that directly patterns nanopores with circular cross sections in series and in plane with the substrate. A focused ion beam (FIB) instrument is used to mill a lamella in a nanochannel and, subsequently, bore a nanopore through the lamella. The diameter and geometry of the nanopore are controlled by the current and dose of the ion beam and by the tilt angle and thickness of the lamella. We fabricated devices with vertical and tilted lamellae and nanopores with diameters from 40 to 90 nm in cylindrical and conical geometries. To test device performance, we conducted resistive-pulse measurements of hepatitis B virus (HBV) capsids. Current pulses from T = 3 capsid (~31 nm diameter) and T = 4 capsid (~35 nm diameter) were well resolved and exhibited relative pulse amplitudes (Δi/i) up to 5 times higher than data obtained on nanopores with rectangular cross sections. For smaller pore diameters (≤ 45 nm), which approach the diameters of the capsids, a dramatic increase in the pulse amplitude was observed for both T = 3 and T = 4 capsids. Two and three pores fabricated in series further improved the resolution between the relative pulse amplitude distributions for the T = 3 and T = 4 capsids by up to 2-fold.
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影响因子：
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影响因子：
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