High JC virus load in tongue carcinomas may be a risk factor for tongue tumorigenesis.
High JC virus load in tongue carcinomas may be a risk factor for tongue tumorigenesis.
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DOI：
10.1007/s00428-007-0534-0
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发表时间：
2008-04
期刊：
影响因子：
--
通讯作者：
Takano Y
中科院分区：
文献类型：
--
作者：
Kutsuna T;Zheng H;Abdel-Aziz HO;Murai Y;Tsuneyama K;Furuta I;Takano Y
John Cunningham病毒（JCV）无症状感染全球大部分人群（约90%），但可能在免疫缺陷患者中被激活，导致进行性多灶性白质脑病。最近的报道表明其在恶性肿瘤中的致癌作用。本文应用实时荧光定量PCR（real-time polymerase chain reaction，PCR）、原位PCR和免疫组化技术检测了39例舌癌、15例舌上皮不典型增生和15例舌炎组织中JCV靶向T抗原的表达，并结合临床病理参数分析了JCV的拷贝数。结果表明，舌炎和DTEs的JCV拷贝数分别为410.5 ± 44.3和658.3 ± 53.3拷贝/μ gDNA，显著低于TC（981.5 ± 14.0，P <0.01）。 < 0.05）。当将它们分为三组时，0-200拷贝/μg DNA（低），201- 1，000拷贝/μg DNA（低），（中度）和大于1001（高度），TC显示3（7.6%）低剂量组，21舌炎发生率低组11例（73.3%），中组0例（0%），高组4例（26.6%）。DTE位于两者之间的中间位置（p < 0.001）。原位PCR显示TC和DTE细胞核呈散在的T抗原阳性，而鼻甲上皮细胞核不呈阳性。T抗原蛋白免疫组化显示4例阳性病例仅在TC。JCV T抗原DNA的存在与TC的临床病理变量无关。结论：JCV的存在可能是舌癌发生的一个危险因素。
The John Cunningham virus (JCV) asymptomatically infects a large proportion (~90%) of the population worldwide but may be activated in immunodeficient patients, resulting in progressive multifocal leukoencephalopathy. Recent reports demonstrated its oncogenic role in malignancies. In this paper, the presence of JCV-targeting T antigen was investigated in tongue carcinoma (TC, n = 39), dysplastic tongue epithelium (DTE, n = 15) and glossitis (n = 15) using real-time polymerase chain reaction (PCR) and in situ PCR and immunohistochemistry, and JCV copies were analyzed with the clinicopathological parameters of TCs. The results demonstrated that glossitis and DTEs had significantly lower copies of JCV (410.5 ± 44.3 and 658.3 ± 53.3 copies/μg DNA respectively) than TCs (981.5 ± 14.0, p < 0.05). When they were divided into three groups with 0–200 copies/μg DNA (low), 201–1,000 (moderate) and more than 1001 (high), TCs showed 3 (7.6%) in the low group, 21 (53.8%) in the moderate group and 15 (38.4%) in the high group and glossitis showed 11 (73.3%) in the low group, 0 (0%) in the moderate group and 4 (26.6%) in the high group. The DTEs occupied an intermediate position between them (p < 0.001). In situ PCR demonstrated that the nuclei of TC and DTE cells are sporadically T-antigen positive but not in nasal turbinate epithelial cells. Immunohistochemistry for T-antigen protein revealed four positive cases only in TCs. The existence of JCV T-antigen DNA was not associated with the clinicopathological variables of TCs. In conclusion, the presence of JCV may be a risk factor of tongue carcinogenesis.
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5.7
作者：
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