Comparative analysis of human brain organoids of brainstem and midbrain at single-cell resolution
Comparative analysis of human brain organoids of brainstem and midbrain at single-cell resolution
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单细胞分辨率下人脑脑干和中脑类器官的比较分析
DOI：
10.1101/2020.09.02.279380
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Mori Eiichiro
中科院分区：
文献类型：
--
作者：
Kinugawa Kaoru;Luginbuhl Joachim;Matsui Takeshi K.;Eura Nobuyuki;Sakaguchi Yoshihiko M.;Shin Jay W;Sugie Kazuma;Schwamborn Jens C.;Mori Eiichiro
人脑类器官为我们研究人脑发育和神经系统疾病提供了手段，单细胞rna测序（scRNA-seq）技术使我们能够识别同源细胞类型和单个细胞之间的分子异质性。通过scRNA-seq分析了先前建立的脑干（hBSOs）和中脑（hMBOs）的人脑类器官，但这些类器官之间的细胞组成差异尚不清楚。在这里，我们整合并比较了hbso和hMBOs的单细胞转录组。我们的分析表明hbso和hmbo含有一些独特的细胞类型，包括炎症细胞和间充质细胞。将hbso和hMBOs与公开的人类胎儿中脑（hMB） scRNA-seq数据集进行进一步比较，发现它们的神经元成分高度相似。这些结果为人类大脑类器官技术提供了新的见解。
Human brain organoids provide us the means to investigate human brain development and neurological diseases, and single-cell RNA-sequencing (scRNA-seq) technologies allow us to identify homologous cell types and the molecular heterogeneity between individual cells. Previously established human brain organoids of brainstem (hBSOs) and midbrain (hMBOs) were analyzed by scRNA-seq, but the difference in cellular composition between these organoids remains unclear. Here, we integrated and compared the single-cell transcriptome of hBSOs and hMBOs. Our analysis demonstrated that the hBSOs and hMBOs contain some unique cell types, including inflammatory and mesenchymal cells. Further comparison of the hBSOs and hMBOs with publicly available scRNA-seq dataset of human fetal midbrain (hMB) showed high similarity in their neuronal components. These results provide new insights into human brain organoid technologies.
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64.5
作者：
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23.9
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