Muscle reinnervation with nerve-muscle-endplate band grafting technique: correlation between force recovery and axonal regeneration.
Muscle reinnervation with nerve-muscle-endplate band grafting technique: correlation between force recovery and axonal regeneration.
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DOI：
10.1016/j.jss.2015.01.008
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发表时间：
2015-05-01
影响因子：
2.2
通讯作者：
Mu, Liancai
中科院分区：
文献类型：
--
作者：
Sobotka, Stanislaw;Mu, Liancai
关键词：
本研究旨在确定在大鼠模型中使用我们最近开发的神经肌肉终板带移植（NMEG）技术进行肌肉再神经支配后功能恢复与轴突再生程度之间的相关性。通过神经横断而实验性瘫痪的右侧胸骨乳突肌 (SM) 立即用从邻近胸骨舌骨肌 (SH) 肌肉获取的 NMEG 蒂重新神经支配。 NMEG 蒂包含肌肉块 (6x6x3 mm)、带有神经末梢的供体神经分支和运动终板带。术后三个月，测量SM肌的强直力，并使用神经丝免疫组化检测肌肉中再生的轴突。结果显示，NMEG 重新神经支配的 SM 肌肉的最大强直力（肌肉功能恢复的衡量标准）达到正常对照的 66.0%。重新神经支配的 SM 肌肉的湿重（肌肉质量恢复的衡量标准）是对照的 87.2%。 NMEG 重新神经支配的 SM 肌肉内通过神经丝染色观察到的再生轴突的面积分数（肌肉重新神经支配的测量）为 42.3%。肌肉神经支配程度与最大肌力之间呈正相关。我们新开发的 NMEG 技术可实现令人满意的功能结果和神经再生。 NMEG 神经支配后功能恢复的进一步改善可以通过改进手术程序并创造有利于轴突终板连接并加速轴突生长和萌芽的理想环境来实现。
This study was designed to determine the correlation between functional recovery and the extent of axonal regeneration after muscle reinnervation with our recently developed nerve-muscle-endplate band grafting (NMEG) technique in a rat model. The right experimentally paralyzed sternomastoid (SM) muscle by nerve transection was immediately reinnervated with a NMEG pedicle harvested from a neighboring sternohyoid (SH) muscle. The NMEG pedicle contained a muscle block (6x6x3 mm), a donor nerve branch with nerve terminals and a motor endplate band. Three months after surgery, the tetanic force of the SM muscle was measured and the regenerated axons in the muscle were detected using neurofilament immunohistochemistry. The results showed that the maximal tetanic force (a measure of muscle functional recovery) of the NMEG reinnervated SM muscle reached up to 66.0% of the normal control. The wet weight of the reinnervated SM muscle (a measure of muscle mass recovery) was 87.2% of the control. The area fraction of the regenerating axons visualized with neurofilament staining within the NMEG reinnervated SM muscle (a measure of muscle reinnervation) was 42.3%. A positive correlation was revealed between the extent of muscle reinnervation and maximal muscle force. Our newly developed NMEG technique results in satisfactory functional outcomes and nerve regeneration. Further improvement in the functional recovery after NMEG reinnervation, could be achieved by refining the surgical procedure and creating an ideal environment that favors axon-endplate connections and accelerates axonal growth and sprouting.
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影响因子：
3.4
作者：
GREEN, DC;BERKE, GS;GRAVES, MC
通讯作者：
GRAVES, MC
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通讯作者：
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作者：
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通讯作者：
Kouyoumdjian, Joao Aris