Improved method of detecting the ERG gene rearrangement in prostate cancer using combined dual-color chromogenic and silver in situ hybridization.
Improved method of detecting the ERG gene rearrangement in prostate cancer using combined dual-color chromogenic and silver in situ hybridization.
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双色显色和银原位杂交联合检测前列腺癌ERG基因重排的改进方法
DOI：
10.1016/j.jmoldx.2012.01.017
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Perner S
中科院分区：
文献类型：
--
作者：
Braun M;Stomper J;Boehm D;Vogel W;Scheble VJ;Wernert N;Shaikhibrahim Z;Fend F;Kristiansen G;Perner S
最近检测到的TMPRSS 2-ERG融合基因被发现是一种复发和流行的前列腺癌（PCa）特异性事件，可能使其有资格用于临床。为了检测这种变化，荧光原位杂交（FISH）是首选的方法。然而，FISH在临床实践中的广泛采用具有一些缺点。随后，显色素原位杂交，它使用有机色原，和酶的金相银原位杂交已成为有前途的亮场替代品。与显色素原位杂交相比，银蛋白原位杂交信号在信号清晰度和分辨率方面非常明显和上级，但该方法排除了荧光探针方案。在ERG断裂FISH技术的基础上，我们建立了一种联合显色素原位杂交和银染原位杂交的双色ERG断裂技术（CS-ISH），并与FISH技术的结果进行了比较。我们对178例前列腺癌和10例良性前列腺癌的连续切片进行双色FISH和CS-ISHERG分离检测，评估其ER重排状态。我们观察到FISH和CS-ISH结果之间的高度显著一致性（97.7%）（Pearson相关系数= 0.955，P < 0.001）。我们的研究结果表明，ER重排状态可以可靠地评估CS-ISH。此外，CS-ISH技术结合了FISH的准确性和精确度与明场显微镜的便利性。该工具允许更广泛的应用，其中研究PCa中ER重排的生物学作用和临床应用。
The recently detectedTMPRSS2-ERGfusion gene was revealed as a recurrent and prevalent prostate cancer (PCa)-specific event, potentially qualifying it for clinical use. To detect this alteration, fluorescencein situhybridization (FISH) is the method of choice. However, FISH has some disadvantages for widespread adoption in clinical practice. Subsequently, chromogenicin situhybridization, which uses organic chromogens, and enzymatic metallography silverin situhybridization have emerged as promising bright-field alternatives. Compared with chromogenicin situhybridization, silverin situhybridization signals are very distinct and superior with regard to signal clarity and resolution, but the method excludes multicolor protocols. Based on theERGbreak-apart FISH assay, we established a dual-colorERGbreak-apart assay using combined chromogenicin situhybridization and silverin situhybridization (CS-ISH) and compared these results with those obtained by FISH. We assessed 178 PCa and 10 benign specimens for theirERGrearrangement status by applying dual-color FISH and CS-ISHERGbreak-apart assays to consecutive sections. We observed a highly significant concordance (97.7%) between FISH- and CS-ISH–based results (Pearson's correlation coefficient = 0.955,P< 0.001). Our findings demonstrate that theERGrearrangement status can reliably be assessed by CS-ISH. Further, the CS-ISH technique combines the accuracy and precision of FISH with the ease of bright-field microscopy. This tool allows a much broader spectrum of applications in which to study the biological role and clinical use ofERGrearrangements in PCa.
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影响因子：
11.2
作者：
Mehra, Rohit;Tomlins, Scott A.;Chinnaiyan, Arul M.
通讯作者：
Chinnaiyan, Arul M.
用于检测 HER2 基因扩增的新型明场分子形态学方法
影响因子：
3.2
作者：
R. Tubbs;J. Pettay;D. Hicks;M. Skacel;R. Powell;T. Grogan;J. Hainfeld
通讯作者：
J. Hainfeld
影响因子：
2.1
作者：
Perner S;Svensson MA;Hossain RR;Day JR;Groskopf J;Slaughter RC;Jarleborn AR;Hofer MD;Kuefer R;Demichelis F;Rickman DS;Rubin MA
通讯作者：
Rubin MA
DOI：
10.1038/modpathol.2010.87
发表时间：
2010-08
期刊：
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc
影响因子：
--
作者：
通讯作者：
--
影响因子：
4.8
作者：
Park, Kyung;Tomlins, Scott A.;Rubin, Mark A.
通讯作者：
Rubin, Mark A.