Identification of neural cells activated by mating stimulus in the periaqueductal gray in female rats.
Identification of neural cells activated by mating stimulus in the periaqueductal gray in female rats.
复制标题
DOI：
10.3389/fnins.2014.00421
复制
发表时间：
2014
影响因子：
4.3
通讯作者：
Kawata M
中科院分区：
文献类型：
--
作者：
Yamada S;Kawata M
啮齿类动物典型的雌性性行为前凸的诱导依赖于雄性动物的大量刺激和血液中雌激素水平。中脑导水管周围灰质（PAG）传出神经元已被认为是重要的脊柱前凸行为，然而，神经化学基础仍有待了解。在这项研究中，我们从神经解剖学角度研究了（1）交配刺激激活的PAG神经元是否投射到延髓网状结构（MRF），这也是脊柱前凸所需的区域;（2）这些神经元是否是神经元。交配刺激显著增加PAG中cFos免疫反应（ir）神经元的数量，特别是在其外侧区。在侧PAG中的cFos-ir神经元的一半是阳性的逆行示踪剂（FluoroGold; FG）注射到MRF。cFos-ir神经元还与囊泡谷氨酸转运体2（vGLUT 2）的mRNA共定位，vGLUT 2是谷氨酸能神经元的分子标记。采用逆行追踪和原位杂交结合荧光显微镜观察，我们还发现FG和vGLUT 2 mRNA双阳性神经元在外侧PAG。这些结果表明，PAG外侧的多巴胺能神经元投射到MRF，并参与了雌性大鼠的脊柱前凸行为。
Induction of lordosis as typical female sexual behavior in rodents is dependent on a mount stimulus from males and blood levels of estrogen. Periaqueductal gray (PAG) efferent neurons have been suggested to be important for lordosis behavior; however, the neurochemical basis remains to be understood. In this study, we neuroanatomically examined (1) whether PAG neurons activated by mating stimulus project to the medullary reticular formation (MRF), which is also a required area for lordosis; and (2) whether these neurons are glutamatergic. Mating stimulus significantly increased the number of cFos-immunoreactive (ir) neurons in the PAG, particularly in its lateral region. Half of cFos-ir neurons in the lateral PAG were positive for a retrograde tracer (FluoroGold; FG) injected into the MRF. cFos-ir neurons also colocalized with mRNA of vesicular glutamate transporter 2 (vGLUT2), a molecular marker for glutamatergic neurons. Using retrograde tracing and in situ hybridization in conjunction with fluorescent microscopy, we also found FG and vGLUT2 mRNA double-positive neurons in the lateral PAG. These results suggest that glutamatergic neurons in the lateral PAG project to the MRF and are involved in lordosis behavior in female rats.
登录
查看更多内容
影响因子：
4.8
作者：
Calizo, LH;Flanagan-Cato, LM
通讯作者：
Flanagan-Cato, LM
DOI：
10.1016/s0169-328x(02)00173-0
发表时间：
2002-04-30
期刊：
MOLECULAR BRAIN RESEARCH
影响因子：
--
作者：
Matsuda, K;Kamiya, Y;Yuzaki, M
通讯作者：
Yuzaki, M
影响因子：
56.9
作者：
HARLAN, RE;SHIVERS, BD;PFAFF, DW
通讯作者：
PFAFF, DW
影响因子：
2.9
作者：
GARGIULO, PA;DONOSO, AO
通讯作者：
DONOSO, AO
影响因子：
2.9
作者：
PFAUS, JG;KLEOPOULOS, SP;PFAFF, DW
通讯作者：
PFAFF, DW