Use of a non-homologous end-joining-deficient strain (delta-ku70) of the biocontrol fungus Trichoderma virens to investigate the function of the laccase gene lcc1 in sclerotia degradation.
Use of a non-homologous end-joining-deficient strain (delta-ku70) of the biocontrol fungus Trichoderma virens to investigate the function of the laccase gene lcc1 in sclerotia degradation.
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DOI：
10.1007/s00294-010-0322-2
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发表时间：
2011-02
期刊：
影响因子：
2.5
通讯作者：
Seidl-Seiboth, Verena
中科院分区：
文献类型：
--
作者：
Catalano, Valentina;Vergara, Mariarosaria;Hauzenberger, Jasmin R.;Seiboth, Bernhard;Sarrocco, Sabrina;Vannacci, Giovanni;Kubicek, Christian P.;Seidl-Seiboth, Verena
利用绿色木霉（Trichoderma virens）产生的Δtku70菌株研究漆酶对植物病原真菌菌核的降解作用。非同源末端连接途径的失活已成为丝状真菌中克服基因工程靶向效率差的成功工具。本文将这一原理应用于生防真菌T. virens，菌株I10，通过删除其tku70基因。该菌株随后被用来删除漆酶基因lcc1，我们发现该基因在T.具有植物病原真菌灰葡萄孢和核盘菌的菌核的病毒。Lcc 1在B的早期定殖中强烈上调。在定殖过程中，能稳定诱导灰葡萄菌核的形成。核盘菌Δtku70Δ lcc 1突变体降解B的菌核的能力发生了改变。cinerea和S.菌核有趣的是，虽然B的衰减能力。cinerea sclerotia显著降低，降解S.甚至增强了对核盘菌菌核的抑制作用，这表明漆酶LCC 1在这两种类型的菌核的真菌寄生中的不同机制的操作。
The aim of this study was to apply a generated Δtku70 strain with increased homologous recombination efficiency from the mycoparasitic fungus Trichoderma virens for studying the involvement of laccases in the degradation of sclerotia of plant pathogenic fungi. Inactivation of the non-homologous end-joining pathway has become a successful tool in filamentous fungi to overcome poor targeting efficiencies for genetic engineering. Here, we applied this principle to the biocontrol fungus T. virens, strain I10, by deleting its tku70 gene. This strain was subsequently used to delete the laccase gene lcc1, which we found to be expressed after interaction of T. virens with sclerotia of the plant pathogenic fungi Botrytis cinerea and Sclerotinia sclerotiorum. Lcc1 was strongly upregulated at early colonization of B. cinerea sclerotia and steadily induced during colonization of S. sclerotiorum sclerotia. The Δtku70Δlcc1 mutant was altered in its ability to degrade the sclerotia of B. cinerea and S. sclerotiorum. Interestingly, while the decaying ability for B. cinerea sclerotia was significantly decreased, that to degrade S. sclerotiorum sclerotia was even enhanced, suggesting the operation of different mechanisms in the mycoparasitism of these two types of sclerotia by the laccase LCC1.
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影响因子：
4.1
作者：
Meyer, Vera;Arentshorst, Mark;Ram, Arthur F. J.
通讯作者：
Ram, Arthur F. J.
影响因子：
4.9
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Haarmann, Thomas;Lorenz, Nicole;Tudzynski, Paul
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Tudzynski, Paul
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通讯作者：
Krappmann, Sven
影响因子：
--
作者：
Krappmann, S;Sasse, C;Braus, GH
通讯作者：
Braus, GH