The COGITAT holeboard system as a valuable tool to assess learning, memory and activity in mice
The COGITAT holeboard system as a valuable tool to assess learning, memory and activity in mice
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COGITAT 孔板系统是评估小鼠学习、记忆和活动的宝贵工具
DOI：
10.1016/j.bbr.2011.01.054
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发表时间：
2011
影响因子：
2.7
通讯作者：
Reif A
中科院分区：
文献类型：
--
作者：
Post A;Wultsch T;Popp S;Painsipp E;Wetzstein H;Kittel-Schneider S;Sontag T;Lesch KP;Reif A
对学习和记忆的各个方面进行全面和无压力的评估是评估神经精神疾病如阿尔茨海默病或注意力缺陷/多动障碍（ADHD）的小鼠模型的先决条件。COGITAT是一种自动化的孔板系统，可以同时评估空间工作和参考记忆的性能，我们在这项研究中已经调整，使其能够用于小鼠。洞板装置由具有25孔地板插入物的开放场地室组成，每个洞由位于三个不同水平上的红外光束监测，允许区分洞的访问，即动物到达洞的底部，或检查，这意味着仅对洞进行表面探索。在整个试验中，动物学会了五个诱饵洞的模式。在这里，我们表明，C57 BL/6小鼠很容易获得这个任务在5天内，每天提交6次试验。一些个人参数-整体探索活动，访问或检查孔的数量，访问或检查的诱饵，未诱饵或以前诱饵的孔的数量，重新检查或重新访问任何孔，吃的小球数量，找到小球的时间，以及参考和工作记忆错误-同时获得，每次实验结束后立即获得结果。毒蕈碱拮抗剂东莨菪碱损害任务的表现，而认知增强剂metrifonate（乙酰胆碱酯酶抑制剂）降低错误率。总的来说，我们的数据表明，这种空间学习任务将是有用的，以表征空间记忆在各种遗传或药理学小鼠模型。
The comprehensive and stress-free assessment of various aspects of learning and memory is a prerequisite to evaluate mouse models for neuropsychiatric disorders such as Alzheimer's disease or attention deficit/hyperactivity disorder (ADHD). COGITAT is an automated holeboard system allowing simultaneous assessment of spatial working and reference-memory performance which we have adapted in this study to enable its usage with mice. The holeboard apparatus consists of an open-field chamber with a 25-hole floor insert, each hole being monitored by infrared light beams, located on three different levels, allowing the distinction between visits of holes, i.e. the animal reaches the bottom of the hole, or inspections, which means only superficial exploration of the hole. Across trials, animals learn a pattern of five baited holes. Here, we show that C57BL/6 mice readily acquire this task within 5 days when submitted to six trials per day. A number of individual parameters – overall exploratory activity, number of visits into or inspections of holes, number of baited, unbaited, or previously baited holes visited or inspected, reinspections of or revisits into any holes, number of pellets eaten, time to find pellets, and reference and working memory errors—are obtained simultaneously and results are immediately available after the end of each experiment. The muscarinic antagonist scopolamine impaired task performance, while the cognitive enhancer metrifonate (an acetylcholinesterase inhibitor) reduced error rates. Overall, our data indicate that this spatial learning task will be useful to characterize spatial memory in various genetic or pharmacological mouse models.
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影响因子：
4
作者：
IDROBO, F;NANDY, K;NANDY, L
通讯作者：
NANDY, L
认知表现中的胆碱能-多巴胺能相互作用。
DOI：
10.1016/0163-1047(90)90639-n
发表时间：
1990
期刊：
Behavioral and neural biology
影响因子：
--
作者：
Levin,ED;McGurk,SR;Rose,JE;Butcher,LL
通讯作者：
Butcher,LL
影响因子：
1.9
作者：
Eleore, Lyndell;Lopez-Ramos, Juan C.;Delgado-Garcia, Jose M.
通讯作者：
Delgado-Garcia, Jose M.
甲曲膦酸钠治疗阿尔茨海默病认知缺陷
影响因子：
9.9
作者：
J. Cummings;P. Cyrus;F. Bieber;J. Mas;J. Orazem;B. Gulanski
通讯作者：
B. Gulanski
大鼠食物搜索行为的发展：海马损伤和氟哌啶醇的影响。
DOI：
--
发表时间：
1978
期刊：
Behavioral Biology
影响因子：
--
作者：
R. Oades;R. Isaacson
通讯作者：
R. Isaacson