Local and Systemic Response to Heterogeneous Sulfate Resupply after Sulfur Deficiency in Rice.
Local and Systemic Response to Heterogeneous Sulfate Resupply after Sulfur Deficiency in Rice.
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DOI：
10.3390/ijms23116203
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发表时间：
2022-05-31
影响因子：
5.6
通讯作者：
Huang, Xin-Yuan
中科院分区：
文献类型：
--
作者：
Wang, Ru-Yuan;Liu, Li-Han;Zhao, Fang-Jie;Huang, Xin-Yuan
硫(S)是植物生长发育所必需的矿质营养物质。植物通常面临硫有效性的时空变化，包括土壤中硫酸盐含量的异质性。作为固着生物，植物已经进化出复杂的机制来修改它们的基因表达和生理过程，以优化S的获取和利用。这种可塑性依赖于一个复杂的网络来局部感知S的可用性，并系统地回应S的状态，这一点仍然知之甚少。在这里，我们利用分根系统，对S缺乏硫酸盐补给的水稻植株进行了转录组范围的基因表达分析。S缺乏分别改变了6749和1589个基因在根和地上部的表达，分别占总转录量的18.07%和4.28%。均质硫酸盐在两半分根中恢复了27.06%的S缺乏症响应基因的表达，而异质硫酸盐补给只恢复了一个分根一半的S缺乏症响应基因的20.76%的表达。在根中发现了局部硫酸盐再供应反应基因，其表达仅在硫酸盐补给的裂根一半中恢复，而在S缺乏的另一半中不表达，主要集中在细胞氨基酸代谢过程和根的生长发育中。在根中也发现了几个系统反应基因，在硫酸盐补给的裂根一半中，这些基因的表达没有变化，但在没有硫酸盐补给的另一半中恢复了表达。系统反应基因主要与钙信号、生长素和ABA信号有关。此外，大量S缺硫响应基因表现出对硫酸盐再供应的局部和系统同步反应，如硫酸盐转运蛋白基因OsSULTR1；1和O-乙酰丝氨酸(硫醇)裂解酶基因，这突显了S缺乏植株在硫酸盐再供应之前吸收和同化硫酸盐的系统调控的存在。我们的研究提供了对异质硫酸盐补给的局部和系统响应的全面的转录组图像，这将有助于理解S在水稻体内稳态的系统调控。
Sulfur (S) is an essential mineral nutrient required for plant growth and development. Plants usually face temporal and spatial variation in sulfur availability, including the heterogeneous sulfate content in soils. As sessile organisms, plants have evolved sophisticated mechanisms to modify their gene expression and physiological processes in order to optimize S acquisition and usage. Such plasticity relies on a complicated network to locally sense S availability and systemically respond to S status, which remains poorly understood. Here, we took advantage of a split-root system and performed transcriptome-wide gene expression analysis on rice plants in S deficiency followed by sulfate resupply. S deficiency altered the expressions of 6749 and 1589 genes in roots and shoots, respectively, accounting for 18.07% and 4.28% of total transcripts detected. Homogeneous sulfate resupply in both split-root halves recovered the expression of 27.06% of S-deficiency-responsive genes in shoots, while 20.76% of S-deficiency-responsive genes were recovered by heterogeneous sulfate resupply with only one split-root half being resupplied with sulfate. The local sulfate resupply response genes with expressions only recovered in the split-root half resupplied with sulfate but not in the other half remained in S deficiency were identified in roots, which were mainly enriched in cellular amino acid metabolic process and root growth and development. Several systemic response genes were also identified in roots, whose expressions remained unchanged in the split-root half resupplied with sulfate but were recovered in the other split-root half without sulfate resupply. The systemic response genes were mainly related to calcium signaling and auxin and ABA signaling. In addition, a large number of S-deficiency-responsive genes exhibited simultaneous local and systemic responses to sulfate resupply, such as the sulfate transporter gene OsSULTR1;1 and the O-acetylserine (thiol) lyase gene, highlighting the existence of a systemic regulation of sulfate uptake and assimilation in S deficiency plants followed by sulfate resupply. Our studies provided a comprehensive transcriptome-wide picture of a local and systemic response to heterogeneous sulfate resupply, which will facilitate an understanding of the systemic regulation of S homeostasis in rice.
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