The speed of tubule formation of two fijiviruses corresponds with their dissemination efficiency in their insect vectors.
The speed of tubule formation of two fijiviruses corresponds with their dissemination efficiency in their insect vectors.
复制标题
两种斐济病毒的小管形成速度与其在昆虫媒介中的传播效率相对应
DOI：
10.1186/s12985-016-0632-1
复制
发表时间：
2016-10-19
期刊：
影响因子：
4.8
通讯作者：
Wei T
中科院分区：
文献类型：
--
作者：
Jia D;Han Y;Sun X;Wang Z;Du Z;Chen Q;Wei T
水稻黑条矮缩病毒（Rice black-streaked dwarf virus，RBSDV）和南方水稻黑条矮缩病毒（Southern rice black-streaked dwarf virus，SRBSDV）是两种关系密切的斐济病毒，分别由小褐飞虱（small brown planthopper，SBPH）和白背飞虱（white backed planthopper，WBPH）传播。SRBSDV的潜伏期比RBSDV短4d，在昆虫媒介中传播效率更高。目前，对这种更高效率的机制知之甚少。然而，我们最近的研究有牵连的作用，病毒诱导的管状结构中的传播fijiiruses在其昆虫vectors.MethodsImmunofluorescence标记进行可视化和比较的P7-1小管形成的RBSDV和SRBSDV在自己的载体昆虫和nonhostSpodoptera frugiperda（Sf 9）cells.ResultsTubule形成的RBSDV P7 -1的速度比RBSDV P7-1。对于RBSDV，在SBPH中第一次接触患病植物（padp）后3天观察到P7-1形成的小管。对于SRBSDV，这些结构早在白背飞虱中1天的padp就被检测到。重要的是，观察到类似的现象时，P7-1蛋白从这两种病毒单独表达在Sf 9 cells.ConclusionsOur研究揭示了P7-1小管形成的速度和病毒传播效率之间的关系，也支持这种管状结构的作用，在昆虫载体内的呼肠孤病毒的传播。
BackgroundRice black-streaked dwarf virus(RBSDV) and Southern rice black-streaked dwarf virus (SRBSDV) are two closely related fijiviruses transmitted by the small brown planthopper (SBPH) and white-backed planthopper (WBPH), respectively. SRBSDV has a latent period 4 days shorter than that of RBSDV, implying a more efficient spread in insect vector. Currently, the mechanisms underlying this higher efficiency are poorly understood. However, our recent studies have implicated a role of virus induced tubular structures in the dissemination of fijiiruses within their insect vectors.MethodsImmunofluorescence labeling was performed to visualize and compare the dynamics of P7-1 tubule formation of the RBSDV and SRBSDV in their own vector insects and nonhostSpodoptera frugiperda(Sf9) cells.ResultsTubule formation of SRBSDV P7-1 was faster than that of RBSDV P7-1. For RBSDV, P7-1 formed tubules were observed at 3-days post-first access to diseased plants (padp) in SBPH. For SRBSDV, these structures were detected as early as 1 day padp in WBPH. Importantly, similar phenomena were observed when P7-1 proteins from the two viruses were expressed alone in Sf9 cells.ConclusionsOur research revealed a relationship between the speed of P7-1 tubule formation and virus dissemination efficiency and also supports a role of such tubular structures in the spread of reoviruses within insect vectors.
登录
查看更多内容
病毒诱导的小管：病毒粒子通过昆虫载体中肠上皮基底层快速传播的载体
影响因子：
5.4
作者：
Jia, Dongsheng;Mao, Qianzhuo;Wei, Taiyun
通讯作者：
Wei, Taiyun
小干扰RNA途径调节昆虫摄入病毒后中肠上皮的初始病毒感染
影响因子：
5.4
作者：
Lan, Hanhong;Chen, Hongyan;Wei, Taiyun
通讯作者：
Wei, Taiyun
植物病毒诱导的管状结构促进病毒在其媒介昆虫中的传播
影响因子：
6.7
作者：
Chen Q;Chen H;Mao Q;Liu Q;Shimizu T;Uehara-Ichiki T;Wu Z;Xie L;Omura T;Wei T
通讯作者：
Wei T
水稻矮缩病毒非结构蛋白Pns10与叶蝉细胞质肌动蛋白之间的相互作用与昆虫载体特异性相关
影响因子：
3.8
作者：
Chen, Qian;Wang, Haitao;Wei, Taiyun
通讯作者：
Wei, Taiyun
开发昆虫载体细胞培养和 RNA 干扰系统以研究斐济病毒复制蛋白的功能作用
影响因子：
5.4
作者：
Jia, Dongsheng;Chen, Hongyan;Wei, Taiyun
通讯作者：
Wei, Taiyun