Tubular structure induced by a plant virus facilitates viral spread in its vector insect.
Tubular structure induced by a plant virus facilitates viral spread in its vector insect.
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植物病毒诱导的管状结构促进病毒在其媒介昆虫中的传播
DOI：
10.1371/journal.ppat.1003032
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发表时间：
2012
期刊：
影响因子：
6.7
通讯作者：
Wei T
中科院分区：
文献类型：
--
作者：
Chen Q;Chen H;Mao Q;Liu Q;Shimizu T;Uehara-Ichiki T;Wu Z;Xie L;Omura T;Wei T
水稻矮缩病毒（RDV）是一种以持续繁殖方式在叶蝉介体中复制和传播的病毒。以前的细胞病理学和遗传学数据显示，管状结构，由非结构性病毒蛋白Pns10构建，包含病毒颗粒，并直接参与RDV在培养的叶蝉细胞之间的细胞间传播。在这里，我们证明了RDV利用这些含病毒的小管移动沿着肌动蛋白为基础的微绒毛的上皮细胞和内脏肌肉组织在消化道中的肌纤维，促进病毒在其昆虫媒介叶蝉体内的传播。在培养的叶蝉细胞中，由Pns10基因合成的dsRNA诱导的RNA干扰（RNAi）导致的Pns10表达的敲低强烈抑制了微管的形成，并阻止了病毒在昆虫载体细胞中的传播。在摄取来自Pns10基因的dsRNA后诱导的RNAi强烈抑制了小管的形成，阻止了叶蝉的细胞间传播和病毒的传播。所有这些结果，第一次表明，一个持续繁殖的病毒利用含病毒的小管组成的非结构性病毒蛋白交通沿着肌动蛋白为基础的细胞突起，促进病毒在载体昆虫的细胞间传播。RNAi技术和昆虫载体细胞培养技术为研究昆虫介导植物病毒高效传播的分子机制提供了有效的工具。
Rice dwarf virus (RDV) replicates in and is transmitted by a leafhopper vector in a persistent-propagative manner. Previous cytopathologic and genetic data revealed that tubular structures, constructed by the nonstructural viral protein Pns10, contain viral particles and are directly involved in the intercellular spread of RDV among cultured leafhopper cells. Here, we demonstrated that RDV exploited these virus-containing tubules to move along actin-based microvilli of the epithelial cells and muscle fibers of visceral muscle tissues in the alimentary canal, facilitating the spread of virus in the body of its insect vector leafhoppers. In cultured leafhopper cells, the knockdown of Pns10 expression due to RNA interference (RNAi) induced by synthesized dsRNA from Pns10 gene strongly inhibited tubule formation and prevented the spread of virus among insect vector cells. RNAi induced after ingestion of dsRNA from Pns10 gene strongly inhibited formation of tubules, preventing intercellular spread and transmission of the virus by the leafhopper. All these results, for the first time, show that a persistent-propagative virus exploits virus-containing tubules composed of a nonstructural viral protein to traffic along actin-based cellular protrusions, facilitating the intercellular spread of the virus in the vector insect. The RNAi strategy and the insect vector cell culture provide useful tools to investigate the molecular mechanisms enabling efficient transmission of persistent-propagative plant viruses by vector insects.
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DOI：
10.1083/jcb.201008173
发表时间：
2011-06-27
期刊：
The Journal of cell biology
影响因子：
--
作者：
Oda H;Takeichi M
通讯作者：
Takeichi M
影响因子：
4.8
作者：
Maroniche GA;Sagadín M;Mongelli VC;Truol GA;del Vas M
通讯作者：
del Vas M
影响因子：
3.7
作者：
Eusebio-Cope, Ana;Sun, Liying;Suzuki, Nobuhiro
通讯作者：
Suzuki, Nobuhiro
影响因子：
3.2
作者：
Honda, Kazuto;Wei, Taiyun;Omura, Toshihiro
通讯作者：
Omura, Toshihiro
开发昆虫载体细胞培养和 RNA 干扰系统以研究斐济病毒复制蛋白的功能作用
影响因子：
5.4
作者：
Jia, Dongsheng;Chen, Hongyan;Wei, Taiyun
通讯作者：
Wei, Taiyun