Recent progress toward the templated synthesis and directed evolution of sequence-defined synthetic polymers.
Recent progress toward the templated synthesis and directed evolution of sequence-defined synthetic polymers.
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DOI：
10.1016/j.chembiol.2009.02.004
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发表时间：
2009-03-27
影响因子：
--
通讯作者：
Liu DR
中科院分区：
文献类型：
--
作者：
Brudno Y;Liu DR
生物聚合物如核酸和蛋白质在生命系统中无处不在，但它们解决自然界中发现的问题之外的问题的能力受到诸如化学或生物不稳定性、有限的构建块功能、生物利用度和免疫原性等因素的限制。原则上，基于非生物单体和主链的序列定义的合成聚合物可以克服这些限制;然而，鉴定具有特定所需功能特性的合成聚合物的序列仍然是一个重大挑战。分子进化可以快速产生功能性聚合物，但需要一种将可生物化的模板（如核酸）翻译成正在进化的聚合物的方法。本文综述了非天然聚合物的酶和非酶模板聚合的最新进展及其在序列定义的合成聚合物定向进化中的潜在应用。
Biological polymers such as nucleic acids and proteins are ubiquitous in living systems, but their ability to address problems beyond those found in nature is constrained by factors such as chemical or biological instability, limited building-block functionality, bioavailability, and immunogenicity. In principle, sequence-defined synthetic polymers based on nonbiological monomers and backbones might overcome these constraints; however, identifying the sequence of a synthetic polymer that possesses a specific desired functional property remains a major challenge. Molecular evolution can rapidly generate functional polymers but requires a means of translating amplifiable templates such as nucleic acids into the polymer being evolved. This review covers recent advances in the enzymatic and nonenzymatic templated polymerization of nonnatural polymers and their potential applications in the directed evolution of sequence-defined synthetic polymers.
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影响因子：
2.9
作者：
Dedkova, Larisa M.;Fahmi, Nour Eddine;Hecht, Sidney M.
通讯作者：
Hecht, Sidney M.
影响因子：
15
作者：
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作者：
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影响因子：
15
作者：
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通讯作者：
DeGrado, WF
影响因子：
3.9
作者：
Chaput, JC;Switzer, C
通讯作者：
Switzer, C