An Assessment of Myotube Morphology, Matrix Deformation, and Myogenic mRNA Expression in Custom-Built and Commercially Available Engineered Muscle Chamber Configurations.
An Assessment of Myotube Morphology, Matrix Deformation, and Myogenic mRNA Expression in Custom-Built and Commercially Available Engineered Muscle Chamber Configurations.
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DOI：
10.3389/fphys.2018.00483
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发表时间：
2018
影响因子：
4
通讯作者：
Mudera V
中科院分区：
文献类型：
--
作者：
Jones JM;Player DJ;Martin NRW;Capel AJ;Lewis MP;Mudera V
文献中报道了几种三维(3D)骨骼肌(SKM)组织工程模型。3D SKM组织工程(TE)的目标是在体外环境中重现天然(活体)组织的结构和功能。这需要将成肌细胞分化为排列整齐的多核肌管，周围环绕着具有生物学代表性的细胞外基质(ECM)。在本工作中，提出了一种新的商品化的由聚醚醚酮(PEEK)制成的3D SKM TE培养箱，以促进这些肌管的适当发展。为了评估这些结构中肌管的结果，将形态、基因表达和ECM重塑参数与先前发表的定制模型进行了比较。在新引入的结构和已建立的结构配置之间，在形态和基因表达方面没有观察到显著的差异，这表明无论制造工艺如何，生物重复性都是可以的。然而，使用商用PEEK小室制造的TE SKM在结构附着和基质变形方面都显示出较低的可变性，这可能是由于制造过程中提高了重复性。系统之间的机械差异也可能是造成这种差异的原因，然而，对这些变量的调查超出了调查的范围。虽然比定制型号昂贵，但这些PEEK腔也适合高压灭菌后的多用途。因此，这将支持它的使用超过以前发表的手工培养箱系统，特别是当寻求开发更高吞吐量的系统时，或者当实验成本不是一个因素时。
There are several three-dimensional (3D) skeletal muscle (SkM) tissue engineered models reported in the literature. 3D SkM tissue engineering (TE) aims to recapitulate the structure and function of native (in vivo) tissue, within an in vitro environment. This requires the differentiation of myoblasts into aligned multinucleated myotubes surrounded by a biologically representative extracellular matrix (ECM). In the present work, a new commercially available 3D SkM TE culture chamber manufactured from polyether ether ketone (PEEK) that facilitates suitable development of these myotubes is presented. To assess the outcomes of the myotubes within these constructs, morphological, gene expression, and ECM remodeling parameters were compared against a previously published custom-built model. No significant differences were observed in the morphological and gene expression measures between the newly introduced and the established construct configuration, suggesting biological reproducibility irrespective of manufacturing process. However, TE SkM fabricated using the commercially available PEEK chambers displayed reduced variability in both construct attachment and matrix deformation, likely due to increased reproducibility within the manufacturing process. The mechanical differences between systems may also have contributed to such differences, however, investigation of these variables was beyond the scope of the investigation. Though more expensive than the custom-built models, these PEEK chambers are also suitable for multiple use after autoclaving. As such this would support its use over the previously published handmade culture chamber system, particularly when seeking to develop higher-throughput systems or when experimental cost is not a factor.
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影响因子：
2.3
作者：
Phillips JB
通讯作者：
Phillips JB
DOI：
10.1007/s10856-016-5731-4
发表时间：
2016-07-01
影响因子：
3.7
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
Baar, Keith
DOI：
10.1177/2211068214557813
发表时间：
2015-06
期刊：
Journal of laboratory automation
影响因子：
--
作者：
Nam KH;Smith AS;Lone S;Kwon S;Kim DH
通讯作者：
Kim DH
影响因子：
3.4
作者：
Gillies, Allison R.;Lieber, Richard L.
通讯作者：
Lieber, Richard L.