MicroRNA and mRNA Dysregulation in Astrocytes Infected with Zika Virus.
MicroRNA and mRNA Dysregulation in Astrocytes Infected with Zika Virus.
复制标题
DOI：
10.3390/v9100297
复制
发表时间：
2017-10-14
期刊：
影响因子：
--
通讯作者：
Kobinger GP
中科院分区：
文献类型：
--
作者：
Kozak RA;Majer A;Biondi MJ;Medina SJ;Goneau LW;Sajesh BV;Slota JA;Zubach V;Severini A;Safronetz D;Hiebert SL;Beniac DR;Booth TF;Booth SA;Kobinger GP
寨卡病毒（ZIKV）的流行是一个持续的公共卫生问题。ZIKV是一种黄病毒，据报道与小头畸形症有关，最近在动物模型中的工作证明了该病毒能够穿过胎盘并影响胎儿大脑发育。最近的研究结果表明，该病毒优先感染神经干细胞，从而解除基因表达，细胞周期进程，并增加细胞死亡。然而，神经元干细胞不是唯一易受ZIKV感染的脑细胞，其他脑细胞的感染可能有助于疾病进展。在本文中，我们表征了星形胶质细胞中的ZIKV复制，并分析了感染期间宿主microRNA（miRNA）和转录组的时间变化。我们观察到许多已知参与黄病毒感染的过程的失调，包括参与未折叠蛋白反应途径的基因。此外，许多miRNA均上调，包括miR-30 e-3 p、miR-30 e-5 p和miR-17- 5 p，它们与其他黄病毒感染相关。这项研究强调了可能在ZIKV发病机制中具有重要意义的潜在miRNA。
The Zika virus (ZIKV) epidemic is an ongoing public health concern. ZIKV is a flavivirus reported to be associated with microcephaly, and recent work in animal models demonstrates the ability of the virus to cross the placenta and affect fetal brain development. Recent findings suggest that the virus preferentially infects neural stem cells and thereby deregulates gene expression, cell cycle progression, and increases cell death. However, neuronal stem cells are not the only brain cells that are susceptible to ZIKV and infection of other brain cells may contribute to disease progression. Herein, we characterized ZIKV replication in astrocytes, and profiled temporal changes in host microRNAs (miRNAs) and transcriptomes during infection. We observed the deregulation of numerous processes known to be involved in flavivirus infection, including genes involved in the unfolded protein response pathway. Moreover, a number of miRNAs were upregulated, including miR-30e-3p, miR-30e-5p, and, miR-17-5p, which have been associated with other flavivirus infections. This study highlights potential miRNAs that may be of importance in ZIKV pathogenesis.
登录
查看更多内容
影响因子：
3.8
作者：
Faye O;Freire CC;Iamarino A;Faye O;de Oliveira JV;Diallo M;Zanotto PM;Sall AA
通讯作者：
Sall AA
影响因子：
56.9
作者：
Jopling, CL;Yi, MK;Sarnow, P
通讯作者：
Sarnow, P
影响因子：
5.4
作者：
Komuro, Akihiko;Horvath, Curt M.
通讯作者：
Horvath, Curt M.
影响因子：
3.3
作者：
Bracci-Laudiero, L;Celestino, D;Aloe, L
通讯作者：
Aloe, L
影响因子：
30.3
作者：
Barrows NJ;Campos RK;Powell ST;Prasanth KR;Schott-Lerner G;Soto-Acosta R;Galarza-Muñoz G;McGrath EL;Urrabaz-Garza R;Gao J;Wu P;Menon R;Saade G;Fernandez-Salas I;Rossi SL;Vasilakis N;Routh A;Bradrick SS;Garcia-Blanco MA
通讯作者：
Garcia-Blanco MA