Unraveling the unique butyrate re-assimilation mechanism of Clostridium sp. strain WK and the application of butanol production from red seaweed Gelidium amansii through a distinct acidolytic pretreatment.
Unraveling the unique butyrate re-assimilation mechanism of Clostridium sp. strain WK and the application of butanol production from red seaweed Gelidium amansii through a distinct acidolytic pretreatment.
复制标题
揭示梭菌独特的丁酸再同化机制。
DOI：
10.1016/j.biortech.2021.125939
复制
发表时间：
2021-09
影响因子：
11.4
通讯作者：
Wu Yi-Rui
中科院分区：
文献类型：
--
作者：
Zhang Kan;Hong Ying;Chen Chaoyang;Wu Yi-Rui
藻源生物丁醇的开发利用具有高性价比和环境友好的特点，已成为研究的热点之一。在本研究中，一株溶剂型菌株Clostridiumsp.菌株WK发酵产丁醇13.96g/L，在24 mg/L丁酸盐存在下，葡萄糖的最高产率为0.41g/g。转录分析表明，该菌株的酸再同化主要受Genesbuk-ptbrather tfAB调控，这解释了其独特的表型，包括对丁酸的高度耐受性和不依赖于pH的发酵。此外，还首次建立了丁酸介导的水解液体系，从红藻生物量(Geldium Amansii)中释放还原糖的最高产量为0.35微克/克。最终丁醇含量达到4.48g/L，显著提高了29.9倍。这项工作揭示了菌株WK合成丁醇的非常规代谢途径，并论证了利用海洋资源开发可再生生物燃料的可行性。
Exploration of the algae-derived biobutanol synthesis has become one of the hotspots due to its highly cost-effective and environment-friendly features. In this study, a solventogenic strainClostridiumsp. strain WK produced 13.96 g/L butanol with a maximal yield of 0.41 g/g from glucose in the presence of 24 g/L butyrate. Transcriptional analysis indicated that the acid re-assimilation of this strain was predominantly regulated by genesbuk-ptbrather thanctfAB, explaining its special phenotypes including high butyrate tolerance and the pH-independent fermentation. In addition, a butyric acid-mediated hydrolytic system was established for the first time to release a maximal yield of 0.35 g/g reducing sugars from the red algal biomass (Gelidium amansii). Moreover, 4.48 g/L of butanol was finally achieved with a significant enhancement by 29.9 folds. This work reveals an unconventional metabolic pathway for butanol synthesis in strain WK, and demonstrates the feasibility to develop renewable biofuels from marine resources.
登录
查看更多内容
影响因子：
3
作者：
Sunwoo, In Yung;Nguyen Trung Hau;Kim, Sung-Koo
通讯作者：
Kim, Sung-Koo
通过新分离的梭菌从硫酸预处理的红藻生物质中生产生物丁醇。
影响因子：
2.8
作者：
Hong Ying;Chen Chaoyang;Wu Yi-Rui
通讯作者：
Wu Yi-Rui
影响因子：
15.3
作者：
Nanna Rhein-Knudsen;A. Meyer
通讯作者：
Nanna Rhein-Knudsen;A. Meyer
影响因子：
2.8
作者：
M. Hessami;S. Cheng;R. Ambati;Y. H. Yin;S. Phang
通讯作者：
M. Hessami;S. Cheng;R. Ambati;Y. H. Yin;S. Phang
影响因子：
11.4
作者：
Congcong Lu;Le Yu;S. Varghese;Mingrui Yu;Shangtian Yang
通讯作者：
Congcong Lu;Le Yu;S. Varghese;Mingrui Yu;Shangtian Yang