Poly(2-ethyl-2-oxazoline) as a sieving matrix for SDS-CE.
Poly(2-ethyl-2-oxazoline) as a sieving matrix for SDS-CE.
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DOI：
10.1002/elps.200900107
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发表时间：
2009-12
期刊：
影响因子：
2.9
通讯作者：
Loge, Gary
中科院分区：
文献类型：
--
作者：
Bernard, Richard;Loge, Gary
在6%~12%w/v的聚合物溶液中，研究了在筛分基质中锰含量为500,000 g/mole的聚(2-乙基-2-恶唑啉)用于蛋白质的十二烷基硫酸钠毛细管电泳粒度分离。在10%w/v时，获得了最佳分离效率，对肌红蛋白(MW=18 kDa)的平均分离效率为150,000(±12,000)理论板，对14根毛细管(MW=30 kDa)的碳酸酐酶(MW=30 kDa)的平均分离效率为8,800,000(±2,400,000)理论板，有效长度为80 cm。一些吸附的聚合物被发现在清洁的毛细管中可以有效地减少EOF；然而，在最初的壁面处理后，发现共价连接的聚丙烯酰胺涂层不那么麻烦。两种不同的缓冲体系，(1)25 mM的HEPES和(2)20 mM的Tris和40 mm或60 mm的三烯，分别加入0.05%或0.10%w/v的十二烷基硫酸钠。三三烯总体上比HEPES具有更好的电流稳定性，但分离效率没有明显的改善。这种聚合物具有使用LIF检测的SDS-CE筛分基质所需的所有特性，包括水解性、光学清晰度、低粘度、可接受的亲水性和商业可用性。
The use of poly(2-ethyl-2-oxazoline) with an Mn of 500,000 g/mole in a sieving matrix for SDS capillary electrophoresis size separation of proteins is investigated using polymer solutions with concentrations between 6% and 12% w/v. Optimal separation efficiency is obtained using 10% w/v, with an average separation efficiency of 150,000 (±12,000) theoretical plates observed for myoglobin (MW = 18 kDa) and 8,800,000 (±2,400,000) theoretical plates for carbonic anhydrase (MW = 30 kDa) for fourteen capillaries with an 80-cm effective length. Some adsorbed polymers were found to be effective in reducing EOF when applied to a clean capillary; however, a covalently-attached polyacrylamide coating was found to be much less troublesome after initial wall treatment. Two different buffer systems, (1) 25 mM HEPES and (2) 20 mM Tris with 40 mM or 60 mM tricene, were tried with 0.05% or 0.10% w/v SDS added. Tris-tricene gave generally better current stability than HEPES but with no observed improvement in separation efficiency. This polymer has all the desired characteristics for an SDS-CE sieving matrix using LIF detection, including hydrolytic stability, optical clarity, low viscosity, acceptable hydrophilicity, and commercial availability.
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影响因子：
2.9
作者：
Craig, DB;Polakowski, PM;Dovichi, NJ
通讯作者：
Dovichi, NJ
影响因子：
4.1
作者：
CHIARI, M;NESI, M;PESEK, JJ
通讯作者：
PESEK, JJ
影响因子：
4.1
作者：
Hu, S;Zhang, ZR;Dovichi, NJ
通讯作者：
Dovichi, NJ
DOI：
10.1016/0021-9673(92)87142-u
发表时间：
1992-09-11
期刊：
JOURNAL OF CHROMATOGRAPHY
影响因子：
--
作者：
WU, D;REGNIER, FE
通讯作者：
REGNIER, FE
影响因子：
2.9
作者：
Albarghouthi, MN;Stein, TM;Barron, AE
通讯作者：
Barron, AE