Fabrication and preliminary evaluation of the osteogenic potential for micro-/nano-structured porous BCP ceramics
Fabrication and preliminary evaluation of the osteogenic potential for micro-/nano-structured porous BCP ceramics
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微纳结构多孔BCP陶瓷的制备及成骨潜力初步评价
DOI：
10.1016/j.ceramint.2019.10.213
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发表时间：
2020-03
影响因子：
5.2
通讯作者：
Li Danyang
中科院分区：
文献类型：
--
作者：
Wang Jing;Su Yangyang;Xu Lizhou;Li Danyang
生物材料的纳米界面在促进组织修复和愈合方面显示出优越性。生物陶瓷是用于生物学目的如组织工程的最常用的生物材料之一。具有智能形貌的纳米陶瓷的制造对于调节与这种表面接触的细胞是必不可少的。采用气体发泡法制备了羟基磷灰石/β-磷酸三钙（HA/β-TCP）多孔双相磷酸钙（BCP）陶瓷基体。然后引入水热反应来改变基底的表面形貌，以获得在不同pH值和离子微环境下的纳米级形貌。采用水热法制备了3种具有不同表面形貌、粗糙度、降解性能和相组成的陶瓷（BCP、BCP与纳米针状表面（BNN）、BCP覆盖有中空纳米棒状表面（BHN））。它们都具有大孔和微孔相互连通的孔隙结构。重要的是，纳米尺度的表面结构增加了总孔体积以及微球体积，从而提高了比表面积（SSA）和随后的生物性能。它们的生物学行为也通过一系列关键事件进行了评估，即蛋白质吸附、细胞附着、增殖和成骨分化。因此，我们发现与具有微米颗粒的原始BCP陶瓷相比，中空纳米棒状结构和纳米针状结构陶瓷表面的纳米结晶增强了体外总血清蛋白和BSA的吸附，并且表现出更持久的BSA释放。此外，3种陶瓷均表现出良好的生物相容性，陶瓷的形态对细胞的铺展和形态起着决定性作用，其中BNN表现出最强的促进MSCs向成骨方向分化的作用。
Nanoscale interface of biomaterials exhibits superiorities in promoting tissue repair and healing. Bioceramics are one of the most commonly used biomaterials for biological purposes such as tissue engineering. The fabrication of nanoscale ceramics with smart topography is essential for modulating cells in contact with such surface. In this research, a gas foaming method was employed to fabricate porous biphasic calcium phosphate (BCP) ceramics as substrates with a controllable component of hydroxyapatite/beta tricalcium phosphate (HA/beta-TCP). Hydrothermal reaction was then introduced to modify the surface morphology of the substrates to obtain a nanoscale topography at different pH values and ionic microenvironments. Three kinds of ceramics (BCP; BCP with a nanoneedle-like surface, BNN; BCP covered with a hollow nanorod-like surface, BHN) were prepared with different surface morphologies and roughness, degradation properties and the phase composition during the hydrothermal process. All of them possessed an interconnected pore structure of macropores and micropores. Importantly, the nano-scale surface structure increased the total pore volumes as well as the micropore volumes, thus enhanced the specific surface area (SSA) and subsequent biological performance. Their biological behavior has also been assessed through a series of pivotal events, i.e. protein adsorption, cell attachment, proliferation, and osteogenic differentiation. Consequently, we found the nano-crystallization of the hollow nanorod-like structured and nanoneedle-like structured ceramic surface enhance the adsorption of both total serum proteins and BSA in vitro compared to the original BCP ceramics with micro-sized grains, as well as exhibit a more persistent release of BSA. Additionally, all the three ceramics have displayed excellent biocompatibilities for MSCs, and the morphology of ceramics played a decisive role in cells spreading and morphology, in which BNN showed the strongest effect to facilitate MSCs differentiation towards osteogenesis.
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影响因子：
46.9
作者：
Lane, Steven W.;Williams, David A.;Watt, Fiona M.
通讯作者：
Watt, Fiona M.
影响因子：
8
作者：
Deng Y;Liu X;Xu A;Wang L;Luo Z;Zheng Y;Deng F;Wei J;Tang Z;Wei S
通讯作者：
Wei S
影响因子：
6.7
作者：
Variola F;Brunski JB;Orsini G;Tambasco de Oliveira P;Wazen R;Nanci A
通讯作者：
Nanci A
DOI：
10.1039/c8tb02283f
发表时间：
2018-11
期刊：
Journal of materials chemistry. B
影响因子：
--
作者：
Jing Wang;Xuening Chen;Bo Guo;Xiao Yang;Yong Zhou;Xiangdong Zhu;Kai Zhang;Yujiang Fan;
通讯作者：
Jing Wang;Xuening Chen;Bo Guo;Xiao Yang;Yong Zhou;Xiangdong Zhu;Kai Zhang;Yujiang Fan;
影响因子：
9.7
作者：
Zhang J;Dalbay MT;Luo X;Vrij E;Barbieri D;Moroni L;de Bruijn JD;van Blitterswijk CA;Chapple JP;Knight MM;Yuan H
通讯作者：
Yuan H