A large genomic island allows Neisseria meningitidis to utilize propionic acid, with implications for colonization of the human nasopharynx.
A large genomic island allows Neisseria meningitidis to utilize propionic acid, with implications for colonization of the human nasopharynx.
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DOI：
10.1111/mmi.12664
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发表时间：
2014-07
影响因子：
3.6
通讯作者：
Moir JW
中科院分区：
文献类型：
--
作者：
Catenazzi MC;Jones H;Wallace I;Clifton J;Chong JP;Jackson MA;Macdonald S;Edwards J;Moir JW
脑膜炎奈瑟菌是一种重要的人类病原体，能够在感染后数小时内杀死。它的正常栖息地是成年人的鼻咽。在这里，我们确定了一个基因组岛（prp基因簇）在N。脑膜炎球菌，使这一物种利用丙酸作为补充碳源在生长过程中，特别是在营养不良的生长条件下。prp基因簇编码柠檬酸甲酯循环的酶。新的方面的甲基柠檬酸循环在N。脑膜炎病毒包括一个丙酸激酶，其被纯化和表征，和一个推定的丙酸转运蛋白。该基因组岛在N.脑膜炎，即脑膜炎奈瑟氏菌，主要定植于婴儿而非成人。我们的理由是，拥有prp基因提供了一个代谢优势，N。成人口腔中的脑膜炎，其中富含丙酸产生细菌。来自经典微生物和基于序列的微生物组研究的数据提供了支持N。脑膜炎球菌的定植与丙酸产生菌相关，含prp的奈瑟氏球菌与来自卟啉单胞菌属的丙酸产生菌之间存在强相关性，这可能解释了青少年/成人脑膜炎球菌的定植。脑膜炎
Neisseria meningitidis is an important human pathogen that is capable of killing within hours of infection. Its normal habitat is the nasopharynx of adult humans. Here we identify a genomic island (the prp gene cluster) in N. meningitidis that enables this species to utilize propionic acid as a supplementary carbon source during growth, particularly under nutrient poor growth conditions. The prp gene cluster encodes enzymes for a methylcitrate cycle. Novel aspects of the methylcitrate cycle in N. meningitidis include a propionate kinase which was purified and characterized, and a putative propionate transporter. This genomic island is absent from the close relative of N. meningitidis, the commensal Neisseria lactamica, which chiefly colonizes infants not adults. We reason that the possession of the prp genes provides a metabolic advantage to N. meningitidis in the adult oral cavity, which is rich in propionic acid-generating bacteria. Data from classical microbiological and sequence-based microbiome studies provide several lines of supporting evidence that N. meningitidis colonization is correlated with propionic acid generating bacteria, with a strong correlation between prp-containing Neisseria and propionic acid generating bacteria from the genus Porphyromonas, and that this may explain adolescent/adult colonization by N. meningitidis.
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影响因子：
3.2
作者：
Heurlier, Karin;Thomson, Melanie J.;Moir, James W. B.
通讯作者：
Moir, James W. B.
人类全血和诱变研究中脑膜炎的转录组分析确定了与血液存活有关的毒力因子。
影响因子：
6.7
作者：
Echenique-Rivera H;Muzzi A;Del Tordello E;Seib KL;Francois P;Rappuoli R;Pizza M;Serruto D
通讯作者：
Serruto D
影响因子：
3.7
作者：
Bogaert D;Keijser B;Huse S;Rossen J;Veenhoven R;van Gils E;Bruin J;Montijn R;Bonten M;Sanders E
通讯作者：
Sanders E
影响因子：
2.9
作者：
Horswill, AR;Escalante-Semerena, JC
通讯作者：
Escalante-Semerena, JC
影响因子：
9.4
作者：
CAUGANT, DA;HOIBY, EA;FROHOLM, LO
通讯作者：
FROHOLM, LO