Variability and diversity of nasopharyngeal microbiota in children: a metagenomic analysis.
Variability and diversity of nasopharyngeal microbiota in children: a metagenomic analysis.
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DOI：
10.1371/journal.pone.0017035
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发表时间：
2011-02-28
期刊：
影响因子：
3.7
通讯作者：
Sanders E
中科院分区：
文献类型：
--
作者：
Bogaert D;Keijser B;Huse S;Rossen J;Veenhoven R;van Gils E;Bruin J;Montijn R;Bonten M;Sanders E
鼻咽是许多口腔细菌和潜在呼吸道或侵入性病原体（如肺炎链球菌、流感嗜血杆菌和脑膜炎奈瑟菌）的生态位。平衡的鼻咽（NP）微生物组的紊乱可能与这些病原体的症状性感染有关，主要发生在秋季和冬季。目前尚不清楚季节性感染模式是否与NP微生物群的伴随变化有关。由于幼儿通常容易发生呼吸道和侵入性感染，我们通过对16 S-rRNA基因的V5-V6高变区进行条形码焦磷酸测序来表征96名健康儿童的NP微生物群，并比较了冬季/秋季采样的儿童与春季采样的儿童之间的微生物群组成。生成的约1000000个序列代表了13个分类学门和约250个种级门类型（OTU）。其中变形菌门（64%）、厚壁菌门（21%）、拟杆菌门（11%）、放线菌门（3%）和梭菌门（1.4%）为优势菌门，莫拉菌属（Moraxella）、嗜血杆菌属（Haemophilus）、链球菌属（Streptococcus）、黄杆菌属（Flavobacteria）、柔丝菌属（Dolosigranulum）、棒状杆菌属（Corynebacterium）和奈瑟菌属（Neisseria）为优势菌属。个体间变异性如此之高，以至于在OTU水平上无法定义核心微生物组。微生物群分布随季节变化很大，在秋季/冬季，变形菌门占主导地位（相对丰度（占所有序列的%）：75%对春季的51%）和梭杆菌属（Fusobacteria）（绝对丰度（儿童百分比）：14%，春季为2%），春季以拟杆菌属为主（相对丰度：秋季/冬季为19%对3%，绝对丰度：秋季/冬季为91%对54%）和厚壁菌门。后者的增加主要是由于（Brevi）芽孢杆菌和乳杆菌属物种（绝对丰度：96%，而秋季/冬季为10%），它们与通常与健康生态系统相关的拟杆菌属物种相似。观察到的季节性影响不能归因于最近的抗生素或病毒合并感染。幼儿的NP微生物群是高度多样化的，并且在季节之间表现出不同。这些差异似乎与抗生素使用或病毒合并感染无关。
The nasopharynx is the ecological niche for many commensal bacteria and for potential respiratory or invasive pathogens like Streptococcus pneumoniae, Haemophilus influenzae, and Neisseria meningitidis. Disturbance of a balanced nasopharyngeal (NP) microbiome might be involved in the onset of symptomatic infections with these pathogens, which occurs primarily in fall and winter. It is unknown whether seasonal infection patterns are associated with concomitant changes in NP microbiota. As young children are generally prone to respiratory and invasive infections, we characterized the NP microbiota of 96 healthy children by barcoded pyrosequencing of the V5–V6 hypervariable region of the 16S-rRNA gene, and compared microbiota composition between children sampled in winter/fall with children sampled in spring. The approximately 1000000 sequences generated represented 13 taxonomic phyla and approximately 250 species-level phyla types (OTUs). The 5 most predominant phyla were Proteobacteria (64%), Firmicutes (21%), Bacteroidetes (11%), Actinobacteria (3%) and Fusobacteria (1,4%) with Moraxella, Haemophilus, Streptococcus, Flavobacteria, Dolosigranulum, Corynebacterium and Neisseria as predominant genera. The inter-individual variability was that high that on OTU level a core microbiome could not be defined. Microbiota profiles varied strongly with season, with in fall/winter a predominance of Proteobacteria (relative abundance (% of all sequences): 75% versus 51% in spring) and Fusobacteria (absolute abundance (% of children): 14% versus 2% in spring), and in spring a predominance of Bacteroidetes (relative abundance: 19% versus 3% in fall/winter, absolute abundance: 91% versus 54% in fall/winter), and Firmicutes. The latter increase is mainly due to (Brevi)bacillus and Lactobacillus species (absolute abundance: 96% versus 10% in fall/winter) which are like Bacteroidetes species generally related to healthy ecosystems. The observed seasonal effects could not be attributed to recent antibiotics or viral co-infection. The NP microbiota of young children is highly diverse and appears different between seasons. These differences seem independent of antibiotic use or viral co-infection.
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影响因子：
14.9
作者：
Pruesse E;Quast C;Knittel K;Fuchs BM;Ludwig W;Peplies J;Glöckner FO
通讯作者：
Glöckner FO
DOI：
10.1073/pnas.091062498
发表时间：
2001-04-24
影响因子：
11.1
作者：
Tusher, VG;Tibshirani, R;Chu, G
通讯作者：
Chu, G
影响因子：
48
作者：
Quince, Christopher;Lanzen, Anders;Sloan, William T.
通讯作者：
Sloan, William T.
影响因子：
--
作者：
Brook, I;Gober, AE
通讯作者：
Gober, AE
影响因子：
4.5
作者：
Huse SM;Dethlefsen L;Huber JA;Mark Welch D;Relman DA;Sogin ML
通讯作者：
Sogin ML