Brown adipose tissue blood flow and mass in obesity: a contrast ultrasound study in mice.
Brown adipose tissue blood flow and mass in obesity: a contrast ultrasound study in mice.
复制标题
DOI：
10.1016/j.echo.2013.07.015
复制
发表时间：
2013-12
影响因子：
6.5
通讯作者：
Scherrer-Crosbie, Marielle
中科院分区：
文献类型：
--
作者：
Clerte, Maeva;Baron, David M.;Brouckaert, Peter;Ernande, Laura;Raher, Michael J.;Flynn, Aidan W.;Picard, Michael H.;Bloch, Kenneth D.;Buys, Emmanuel S.;Scherrer-Crosbie, Marielle
当被交感神经系统激活时，棕色脂肪组织（BAT）增加能量消耗以产生热量。增加BAT的质量或增加BAT的激活可能潜在地用于减少肥胖。需要无创方法检测和监测BAT肿块。对比超声可以估计BAT血流量，并能够测量器官的灌注容量，从而测量其质量。本研究的目的是评估对比超声是否可以表征两种肥胖小鼠模型：高脂肪饮食的野生型小鼠和突变db/db小鼠的BAT肿块。去甲肾上腺素（NE）刺激BAT前后行BAT对比超声（清晰度2 μL/min， 14 mhz线性探头）。得到BAT补充曲线，并通过曲线平台与斜率的乘积估计血流量。此外，在NE刺激后每隔1mm获得灌注BAT的连续二维图像，并用于评估BAT的体积和质量。在所有小鼠中，NE输注后BAT血流量增加。在低脂饮食和高脂饮食的野生型小鼠中，对NE的血流反应是相似的。db/db小鼠的BAT血流量低于野生型小鼠（P = 0.02）。超声造影所得BAT肿块与尸检所得BAT肿块相关（R2 = 0.83, P < 0.001）。喂食高脂肪食物的老鼠的BAT质量高于喂食低脂肪食物的老鼠。在BAT血管化未明显受损的情况下，对比超声可用于估计小鼠BAT的质量。这种非侵入性技术可能允许对旨在增加BAT质量的疗法进行系列评估。
When activated by the sympathetic nervous system, brown adipose tissue (BAT) increases energy expenditure to produce heat. Augmenting BAT mass or increasing BAT activation could potentially be used to decrease obesity. Noninvasive methods to detect and monitor BAT mass are needed. Contrast ultrasound can estimate BAT blood flow and is able to measure the perfused volume of an organ and thus its mass. The objective of this study was to evaluate whether contrast ultrasound could characterize BAT mass in two mouse models of obesity: wild-type mice fed a high-fat diet and mutant db/db mice. Contrast ultrasound of BAT (Definity 2 μL/min; 14-MHz linear probe) was performed before and after stimulation of BAT with norepinephrine (NE). BAT replenishment curves were obtained, and blood flow was estimated by the product of the curve’s plateau and slope. Additionally, consecutive two-dimensional images of perfused BAT were acquired at 1-mm intervals after stimulation with NE and used to assess BAT volume and mass. BAT blood flow increased after NE infusion in all mice studied. Blood flow response to NE was similar in wild-type mice fed either a low-fat diet or a high-fat diet. BAT blood flow was lower in db/db mice than in wild-type mice (P = .02). Contrast ultrasound–derived BAT mass was correlated with BAT mass obtained at necropsy (R2 = 0.83, P < .001). BAT mass was higher in mice fed a high-fat diet than in those fed a low-fat diet. Contrast ultrasound can be used to estimate BAT mass in mice when BAT vascularization is not significantly impaired. This noninvasive technique may potentially allow the serial evaluation of therapies designed to augment BAT mass.
登录
查看更多内容
DOI：
10.1152/ajpheart.2000.279.4.h1698
发表时间：
2000-10-01
影响因子：
4.8
作者：
Feldman, MD;Erikson, JM;Freeman, GL
通讯作者：
Freeman, GL
影响因子：
2.1
作者：
FOSTER, DO;FRYDMAN, ML
通讯作者：
FRYDMAN, ML
影响因子：
4.8
作者：
Cinti, S;Frederich, RC;Lowell, BB
通讯作者：
Lowell, BB
影响因子：
6.9
作者：
Chen, Y. Iris;Cypess, Aaron M.;Sass, Christina A.;Brownell, Anna-Liisa;Jokivarsi, Kimmo T.;Kahn, C. Ronald;Kwong, Kenneth K.
通讯作者：
Kwong, Kenneth K.
影响因子：
158.5
作者：
Connolly, HM;Crary, JL;Schaff, HV
通讯作者：
Schaff, HV