Anatomical and functional assessment of brown adipose tissue by magnetic resonance imaging.
Anatomical and functional assessment of brown adipose tissue by magnetic resonance imaging.
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DOI：
10.1038/oby.2012.22
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发表时间：
2012-07
期刊：
影响因子：
6.9
通讯作者：
Kwong, Kenneth K.
中科院分区：
文献类型：
--
作者：
Chen, Y. Iris;Cypess, Aaron M.;Sass, Christina A.;Brownell, Anna-Liisa;Jokivarsi, Kimmo T.;Kahn, C. Ronald;Kwong, Kenneth K.
棕色脂肪组织（BAT）是哺乳动物非颤抖性产热的主要组织。BAT的量及其活化水平有助于调节用于产热的过量卡路里的利用，而不是储存在白色脂肪组织（WAT）中，这将导致体重增加。在过去的几年中，体内BAT活性主要通过正电子发射断层扫描-计算机断层扫描（PET-CT）扫描评估，使用2-[18 F]-氟-2-脱氧-D-葡萄糖（18 F-FDG）测量与BAT线粒体呼吸相关的葡萄糖利用率。在这项研究中，我们证明了可行性的映射和估计BAT的体积和代谢功能在体内大鼠在9.4T磁共振成像（MRI）扫描仪使用序列可从临床MR扫描仪。基于BAT的形态学特征，我们使用具有强脂水对比的MRI序列测量了BAT的体积分布。我们还利用自旋回波MRI序列研究了BAT体积。体内MRI估计的BAT体积与解剖样本中BAT质量的直接测量值相关。使用MRI，我们还能够绘制β3-肾上腺素能受体激动剂CL-316，243诱导的BAT代谢变化的血流动力学反应，并将其与PET 18F-FDG评估的对CL-316，243反应的BAT活性进行比较。总之，我们证明了使用常规MRI序列测量体内BAT体积和功能的可行性。BAT体积的MRI测量与离体组织的定量测量一致。
Brown adipose tissue (BAT) is the primary tissue responsible for nonshivering thermogenesis in mammals. The amount of BAT and its level of activation help regulate the utilization of excessive calories for thermogenesis as opposed to storage in white adipose tissue (WAT) which would lead to weight gain. Over the past several years, BAT activity in vivo has been primarily assessed by positron emission tomography-computed tomography (PET-CT) scan using 2-[18F]-fluoro-2-deoxy-D-glucose (18F-FDG) to measure glucose utilization associated with BAT mitochondrial respiration. In this study, we demonstrate the feasibility of mapping and estimating BAT volume and metabolic function in vivo in rats at a 9.4T magnetic resonance imaging (MRI) scanner using sequences available from clinical MR scanners. Based on the morphological characteristics of BAT, we measured the volume distribution of BAT with MRI sequences that have strong fat–water contrast. We also investigated BAT volume by utilizing spin-echo MRI sequences. The in vivo MRI-estimated BAT volumes were correlated with direct measurement of BAT mass from dissected samples. Using MRI, we also were able to map hemodynamic responses to changes in BAT metabolism induced pharmacologically by β3-adrenergic receptor agonist, CL-316,243 and compare this to BAT activity in response to CL-316,243 assessed by PET 18F-FDG. In conclusion, we demonstrate the feasibility of measuring BAT volume and function in vivo using routine MRI sequences. The MRI measurement of BAT volume is consistent with quantitative measurement of the tissue ex vivo.
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