Characterization of radiation sensitivity of human squamous carcinoma A431 cells.
Characterization of radiation sensitivity of human squamous carcinoma A431 cells.
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人鳞癌 A431 细胞辐射敏感性的表征。
DOI：
10.1038/bjc.1987.193
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发表时间：
1987
影响因子：
8.8
通讯作者：
Sutherland,RM
中科院分区：
文献类型：
--
作者：
Ng,CE;Keng,PC;Sutherland,RM
已经进行了研究以调查人鳞状细胞癌细胞的放射敏感性。A431细胞在体外生长为指数和补料平台单层培养物或多细胞球体。各种培养物的放射生物学研究表明，补料平台期细胞（D 0 = 1.3戈伊）比指数生长期细胞（D 0 = 1.5戈伊）更敏感。A431细胞经单次12戈伊或两次6戈伊照射后，表现出较大的潜在致死性损伤（PLD）修复能力（PLD修复因子= 17），但亚致死性损伤（SLD）修复能力相对较小。为了测量增殖（P）和静止（Q）细胞的辐射敏感性，从A431球状体中分离P-和Q-细胞的富集群体。吖啶橙子（AO）染色的流式细胞术分析表明，在补料平台和球状体培养物中的RNA直方图向低值偏移，表明存在Q细胞亚群。使用离心淘洗从解离的球状体细胞中分离Q细胞。库尔特细胞体积分布和流式细胞仪分析表明，Q-细胞具有小的细胞体积（约1380微米3），低RNA含量和G1样DNA含量。连续标记实验与氚标记的胸苷证实了非增殖性质的Q-细胞。从球状体分离后，用0至10戈伊的Q-细胞的照射显示它们比从这些球状体分离的P-细胞更放射敏感（D 0降低）。然而，后者在放射敏感性指数G1细胞相似。
Studies have been performed to investigate the radiosensitivity of human squamous carcinoma cells. A431 cells were grown in vitro as exponential and fed-plateau monolayer cultures or as multicellular spheroids. Radiobiological studies of various cultures showed that fed-plateau phase cells were more sensitive (D0= 1.3 Gy) than exponentially growing cells (D0= 1.5 Gy). After a single dose of 12 Gy or two doses of 6 Gy irradiation, A431 cultures exhibited a large capacity for potentially lethal damage (PLD) repair (PLD repair factor= 17), but a relatively small sublethal damage (SLD) repair. In order to measure the radiation sensitivity of proliferating (P) and quiescent (Q) cells, enriched populations of P-and Q-cells were isolated from A431 spheroids. Flow cytometric analysis with acridine orange (AO) staining demonstrated that there was a shift of the RNA histograms in fed-plateau and spheroid cultures towards lower values, suggesting the presence of a subpopulation of Q-cells. Centrifugal elutriation was used to isolate the Q-cells from dissociated spheroid cells. Coulter cell volume distributions and flow cytometric analysis showed that Q-cells had a small cell volume (approximately 1380 microns3), low RNA content and a G1-like DNA content. Continuous labelling experiments with tritiated thymidine confirmed the non-proliferating nature of the Q-cells. Irradiation of the Q-cells after isolation from spheroids with between 0 to 10 Gy showed that they were more radiosensitive (decreased D0) than the P-cells isolated from these spheroids. The latter were, however, similar in radiosensitivity to exponential G1 cells.
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影响因子：
11.2
作者：
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通讯作者：
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从多细胞球体中分离出的增殖和静止亚群的辐射反应。
影响因子：
8.8
作者：
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通讯作者：
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影响因子：
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作者：
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DOI：
10.1016/0010-468x(79)90071-0
发表时间：
1979
期刊：
Computer programs in biomedicine
影响因子：
--
作者：
J. Fried;M. Mandel
通讯作者：
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影响因子：
5.6
作者：
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通讯作者：
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