Hsp20 functions as a novel cardiokine in promoting angiogenesis via activation of VEGFR2.
Hsp20 functions as a novel cardiokine in promoting angiogenesis via activation of VEGFR2.
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DOI：
10.1371/journal.pone.0032765
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Fan GC
中科院分区：
文献类型：
--
作者：
Zhang X;Wang X;Zhu H;Kranias EG;Tang Y;Peng T;Chang J;Fan GC
Heat shock proteins (Hsps) are well appreciated as intrinsic protectors of cardiomyocytes against numerous stresses. Recent studies have indicated that Hsp20 (HspB6), a small heat shock protein, was increased in blood from cardiomyopathic hamsters. However, the exact source of the increased circulating Hsp20 and its potential role remain obscure. In this study, we observed that the circulating Hsp20 was increased in a transgenic mouse model with cardiac-specific overexpression of Hsp20, compared with wild-type mice, suggesting its origin from cardiomyocytes. Consistently, culture media harvested from Hsp20-overexpressing cardiomyocytes by Ad.Hsp20 infection contained an increased amount of Hsp20, compared to control media. Furthermore, we identified that Hsp20 was secreted through exosomes, independent of the endoplasmic reticulum-Golgi pathway. To investigate whether extracellular Hsp20 promotes angiogenesis, we treated human umbilical vein endothelial cells (HUVECs) with recombinant human Hsp20 protein, and observed that Hsp20 dose-dependently promoted HUVEC proliferation, migration and tube formation. Moreover, a protein binding assay and immunostaining revealed an interaction between Hsp20 and VEGFR2. Accordingly, stimulatory effects of Hsp20 on HUVECs were blocked by a VEGFR2 neutralizing antibody and CBO-P11 (a VEGFR inhibitor). These in vitro data are consistent with the in vivo findings that capillary density was significantly enhanced in Hsp20-overexpressing hearts, compared to non-transgenic hearts. Collectively, our findings demonstrate that Hsp20 serves as a novel cardiokine in regulating myocardial angiogenesis through activation of the VEGFR signaling cascade.
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影响因子：
5
作者：
Glembotski CC
通讯作者：
Glembotski CC
影响因子：
20.1
作者：
Fan GC;Zhou X;Wang X;Song G;Qian J;Nicolaou P;Chen G;Ren X;Kranias EG
通讯作者：
Kranias EG
影响因子：
13.6
作者：
Doroudgar S;Glembotski CC
通讯作者：
Glembotski CC
影响因子：
5
作者：
Fan GC;Kranias EG
通讯作者：
Kranias EG
外泌体在氧化应激期间传达保护性信息；外泌体穿梭RNA的可能作用。
影响因子：
3.7
作者：
Eldh M;Ekström K;Valadi H;Sjöstrand M;Olsson B;Jernås M;Lötvall J
通讯作者：
Lötvall J