Exosomes communicate protective messages during oxidative stress; possible role of exosomal shuttle RNA.
Exosomes communicate protective messages during oxidative stress; possible role of exosomal shuttle RNA.
复制标题
外泌体在氧化应激期间传达保护性信息；外泌体穿梭RNA的可能作用。
DOI：
10.1371/journal.pone.0015353
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发表时间：
2010-12-17
期刊：
影响因子：
3.7
通讯作者：
Lötvall J
中科院分区：
文献类型：
--
作者：
Eldh M;Ekström K;Valadi H;Sjöstrand M;Olsson B;Jernås M;Lötvall J
胞外体是一种小的细胞外纳米囊泡，来源于内源性，通过表面相互作用和将功能RNA从一个细胞运送到另一个细胞，在细胞之间传递不同的信号。Exosome由许多细胞释放，包括肥大细胞、树突状细胞、巨噬细胞、上皮细胞和肿瘤细胞。Exosome与它们的供体细胞相比，不仅在大小上不同，而且在RNA、蛋白质和脂肪组成上也不同。在这项研究中，我们发现暴露在氧化应激下的小鼠肥大细胞释放的外切体在其mRNA含量上有所不同。此外，我们还表明，这些外切体可以通过为受体细胞提供对氧化应激的抵抗力来影响其他细胞对氧化应激的反应，氧化应激被观察到是细胞活力的减弱损失。此外，Affymetrix微阵列分析表明，外切体的mRNA含量不仅在外切体和供体细胞之间存在差异，而且在不同条件下生长的细胞的外切体之间也存在差异；氧化应激和正常条件下生长的细胞的外切体之间也存在差异。最后，我们还表明，暴露在紫外光下会影响氧化应激下释放的外切体相关的生物功能。这些结果表明，通过影响受体细胞对外部应激刺激的反应，RNA的外体穿梭参与了细胞间的通讯。
Exosomes are small extracellular nanovesicles of endocytic origin that mediate different signals between cells, by surface interactions and by shuttling functional RNA from one cell to another. Exosomes are released by many cells including mast cells, dendritic cells, macrophages, epithelial cells and tumour cells. Exosomes differ compared to their donor cells, not only in size, but also in their RNA, protein and lipid composition. In this study, we show that exosomes, released by mouse mast cells exposed to oxidative stress, differ in their mRNA content. Also, we show that these exosomes can influence the response of other cells to oxidative stress by providing recipient cells with a resistance against oxidative stress, observed as an attenuated loss of cell viability. Furthermore, Affymetrix microarray analysis revealed that the exosomal mRNA content not only differs between exosomes and donor cells, but also between exosomes derived from cells grown under different conditions; oxidative stress and normal conditions. Finally, we also show that exposure to UV-light affects the biological functions associated with exosomes released under oxidative stress. These results argue that the exosomal shuttle of RNA is involved in cell-to-cell communication, by influencing the response of recipient cells to an external stress stimulus.
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DOI：
10.1007/bf00267850
发表时间：
1979-01-01
期刊：
MOLECULAR AND GENERAL GENETICS
影响因子：
--
作者：
PONTA, H;PFENNIGYEH, ML;HERRLICH, P
通讯作者：
HERRLICH, P
DOI：
10.1083/jcb.101.3.942
发表时间：
1985-09
期刊：
The Journal of cell biology
影响因子：
--
作者：
Pan BT;Teng K;Wu C;Adam M;Johnstone RM
通讯作者：
Johnstone RM
影响因子：
24.3
作者：
Admyre, C;Grunewald, J;Gabrielsson, S
通讯作者：
Gabrielsson, S
影响因子：
5.4
作者：
Admyre, Charlotte;Johansson, Sara M.;Gabrielsson, Susanne
通讯作者：
Gabrielsson, Susanne
影响因子：
2.5
作者：
Chen, ZH;Yoshida, Y;Niki, E
通讯作者：
Niki, E