Characterization of host-dependent mutations of apple fruit crinkle viroid replicating in newly identified experimental hosts suggests maintenance of stem-loop structures in the left-hand half of the molecule is important for replication.
Characterization of host-dependent mutations of apple fruit crinkle viroid replicating in newly identified experimental hosts suggests maintenance of stem-loop structures in the left-hand half of the molecule is important for replication.
复制标题
苹果果实皱纹类病毒在新鉴定的实验宿主中复制的宿主依赖性突变的特征表明，分子左半部分茎环结构的维持对于复制很重要。
DOI：
10.1099/jgv.0.000693
复制
发表时间：
2017
期刊：
影响因子：
--
通讯作者：
T. Sano
中科院分区：
文献类型：
--
作者：
Takahiro Suzuki;Misato Fujibayashi;T. Hataya;A. Taneda;Ying;T. Tsushima;G. Duraisamy;K. Siglová;J. Matoušek;T. Sano
苹果皱果类病毒（AFCVd）是拟痘病毒科Apscaviroidae属的一个新成员。AFCVd具有狭窄的宿主范围，并且已知感染作为天然宿主的苹果、啤酒花和柿子。在这项研究中，番茄，黄瓜和野生啤酒花已被确定为新的实验草本寄主。叶片症状非常轻微或几乎检测不到，但受感染的番茄果实小，破裂和扭曲。这些症状类似于在一些AFCVD敏感的苹果品种上观察到的症状。转移到番茄，黄瓜和野生啤酒花，序列变化检测到一个自然的AFCVd分离的啤酒花，和主要的变异体在番茄，黄瓜和野生啤酒花不同的10，8或2个核苷酸，分别从接种物中的优势。番茄和黄瓜中的主要变异体几乎相同，野生啤酒花中的变异体与栽培啤酒花中的变异体非常相似。使用小RNA深度序列数据和二聚体RNA转录物的感染性研究，随后进行后代分析，对转移到番茄后来自啤酒花的天然存在的AFCVd分离物中出现的宿主依赖性序列变化进行详细分析，表明番茄中的主要AFCVd变体是通过选择接种物（即啤酒花）中存在的次要变体，然后选择一到两个宿主依赖性从头突变而出现的。比较的二级结构的主要变种在啤酒花，番茄和柿子转移到番茄后，表明茎环结构的左半部分的分子的维护是至关重要的感染。
Apple fruit crinkle viroid (AFCVd) is a tentative member of the genus Apscaviroid, family Pospiviroidae. AFCVd has a narrow host range and is known to infect apple, hop and persimmon as natural hosts. In this study, tomato, cucumber and wild hop have been identified as new experimental herbaceous hosts. Foliar symptoms were very mild or virtually undetectable, but fruits of infected tomato were small, cracked and distorted. These symptoms resemble those observed on some AFCVd-sensitive apple cultivars. After transfer to tomato, cucumber and wild hop, sequence changes were detected in a natural AFCVd isolate from hop, and major variants in tomato, cucumber and wild hop differed in 10, 8 or 2 nucleotides, respectively, from the predominant one in the inoculum. The major variants in tomato and cucumber were almost identical, and the one in wild hop was very similar to the one in cultivated hop. Detailed analyses of the host-dependent sequence changes that appear in a naturally occurring AFCVd isolate from hop after transfer to tomato using small RNA deep sequence data and infectivity studies with dimeric RNA transcripts followed by progeny analysis indicate that the major AFCVd variant in tomato emerged by selection of a minor variant present in the inoculum (i.e. hop) followed by one to two host-dependent de novo mutations. Comparison of the secondary structures of major variants in hop, tomato and persimmon after transfer to tomato suggested that maintenance of stem-loop structures in the left-hand half of the molecule is critical for infection.
影响因子：
3.7
作者：
Kawaguchi-Ito Y;Li SF;Tagawa M;Araki H;Goshono M;Yamamoto S;Tanaka M;Narita M;Tanaka K;Liu SX;Shikata E;Sano T
通讯作者：
Sano T
影响因子：
2.7
作者：
Di Serio, F.;Flores, R.;Owens, R. A.
通讯作者：
Owens, R. A.