Effects of Pituitary Adenylate Cyclase‐Activating Polypeptide, Vasoactive Intestinal Polypeptide, and Somatostatin on the Release of Thyrotropin from the Bullfrog Pituitary
Effects of Pituitary Adenylate Cyclase‐Activating Polypeptide, Vasoactive Intestinal Polypeptide, and Somatostatin on the Release of Thyrotropin from the Bullfrog Pituitary
复制标题
垂体腺苷酸环化酶激活多肽、血管活性肠多肽和生长抑素对牛蛙垂体促甲状腺素释放的影响
DOI：
10.1196/annals.1317.064
复制
发表时间：
2006
影响因子：
5.2
通讯作者：
S. Kikuyama
中科院分区：
文献类型：
--
作者：
R. Okada;Kazutoshi Yamamoto;Yoichi Ito;N. Chartrel;J. Leprince;A. Fournier;H. Vaudry;S. Kikuyama
摘要： 最近发展了一种两栖类(牛蛙，牛蛙)促甲状腺激素(TSH)的特异性放射免疫分析方法，使我们能够在体外研究各种神经肽对脑垂体TSH释放的影响。到目前为止，牛蛙来源的促肾上腺皮质激素释放因子被证明具有很强的TSH释放活性，而促性腺激素释放激素和TSH释放激素对成虫的TSH释放活性中等，但对幼虫的TSH释放活性不明显。本文研究了垂体腺苷酸环化酶激活多肽(PACAP)、血管活性肠肽(VIP)和生长抑素(SS)对牛蛙脑垂体促甲状腺激素(TSH)释放的影响。在24小时培养过程中，青蛙(R.ridibunda)PACAP-38和PACAP-27均呈浓度依赖性地刺激分散的垂体细胞释放TSH。同时应用PACAP6-38可抑制PACAP-38和PACAP-27诱导的TSH释放。单独应用高浓度PACAP6-38对TSH的释放有轻微但显著的刺激作用。青蛙VIP还浓度依赖性地刺激垂体细胞释放TSH。蛙类SS1(与哺乳动物生长抑素-14同源)和SS2(与哺乳动物皮质抑素同源)不影响TSH的基础释放，但对PACAP-38诱导的TSH释放有浓度依赖性的抑制作用。这些结果表明，多种神经肽参与了两栖类脑垂体TSH释放的调节。
Abstract: The recent development of a specific radioimmunoassay for amphibian (bullfrog, Rana catesbeiana) thyrotropin (TSH) has made it possible to study the effects of various neuropeptides on the release of TSH from the pituitary in vitro. Up to now, corticotropin‐releasing factor of bullfrog origin has been shown to have a potent TSH‐releasing activity, whereas gonadotropin‐releasing hormone and TSH‐releasing hormone exhibit a moderate TSH‐releasing effect on the adult, but not larval, pituitary. In the present study, the effects of pituitary adenylate cyclase‐activating polypeptide (PACAP), vasoactive intestinal polypeptide (VIP), and somatostatin (SS) on the in vitro release of TSH from the bullfrog pituitary were investigated. Both frog (R. ridibunda) PACAP‐38 and PACAP‐27 caused a concentration‐dependent stimulation of the release of TSH from dispersed pituitary cells during a 24‐h culture. The PACAP‐38‐ and PACAP‐27‐induced TSH release was suppressed by a simultaneous application of PACAP6–38. Application of high concentrations of PACAP6–38 alone caused a slight but significant stimulatory effect on the release of TSH. Frog VIP also stimulated TSH release from pituitary cells concentration‐dependently. Frog SS1 (homologous to mammalian somatostatin‐14) and SS2 (homologous to mammalian cortistatin) did not affect the basal release of TSH but caused a concentration‐dependent suppression of the PACAP‐38‐induced release of TSH. These results suggest the involvement of multiple neuropeptides in the regulation of the release of TSH from the amphibian pituitary.
DOI：
10.1016/0006-291x(90)92140-u
发表时间：
1990-07-31
影响因子：
3.1
作者：
MIYATA, A;JIANG, L;ARIMURA, A
通讯作者：
ARIMURA, A
DOI：
10.1016/0006-291x(89)91757-9
发表时间：
1989-10-16
影响因子：
3.1
作者：
MIYATA, A;ARIMURA, A;COY, DH
通讯作者：
COY, DH