Large-scale screening of transcription factor-promoter interactions in spruce reveals a transcriptional network involved in vascular development.
Large-scale screening of transcription factor-promoter interactions in spruce reveals a transcriptional network involved in vascular development.
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DOI：
10.1093/jxb/eru116
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发表时间：
2014-06
影响因子：
6.9
通讯作者：
Séguin A
中科院分区：
文献类型：
--
作者：
Duval I;Lachance D;Giguère I;Bomal C;Morency MJ;Pelletier G;Boyle B;MacKay JJ;Séguin A
本研究旨在研究不同转录因子（TFs）在针叶树中的作用，并在相对高通量水平上直接描绘基因调控网络。该方法整合了序列分析、转录谱分析和针叶树特异性激活试验的开发。对102个tf和潜在靶基因的转录物积累谱进行聚类，以确定协调表达的基因群。在9个不同的器官和组织中发现了几种不同的转录物积累模式：27个基因在茎和根中都优先于次生木质部，而其他基因则优先于木质部和周皮或更为普遍。已经建立了一个强大的系统作为筛选方法来确定哪些tf有能力调节植物中给定的启动子。在30%的tf候选基因启动子组合中观察到反式激活或抑制作用。为了证明这一概念，系统发育分析、表达和反式激活数据表明，在其他植物物种中观察到的两种云杉nac结构域蛋白很可能在次生维管生长中起关键作用。本研究测试了来自不同科的针叶树的许多tf，拓宽了对木材发育中启动子- tf相互作用的认识，并可以比较被子植物和裸子植物中发现的基因调控网络。
This research aimed to investigate the role of diverse transcription factors (TFs) and to delineate gene regulatory networks directly in conifers at a relatively high-throughput level. The approach integrated sequence analyses, transcript profiling, and development of a conifer-specific activation assay. Transcript accumulation profiles of 102 TFs and potential target genes were clustered to identify groups of coordinately expressed genes. Several different patterns of transcript accumulation were observed by profiling in nine different organs and tissues: 27 genes were preferential to secondary xylem both in stems and roots, and other genes were preferential to phelloderm and periderm or were more ubiquitous. A robust system has been established as a screening approach to define which TFs have the ability to regulate a given promoter in planta. Trans-activation or repression effects were observed in 30% of TF–candidate gene promoter combinations. As a proof of concept, phylogenetic analysis and expression and trans-activation data were used to demonstrate that two spruce NAC-domain proteins most likely play key roles in secondary vascular growth as observed in other plant species. This study tested many TFs from diverse families in a conifer tree species, which broadens the knowledge of promoter–TF interactions in wood development and enables comparisons of gene regulatory networks found in angiosperms and gymnosperms.
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影响因子：
6.9
作者：
Bomal, Claude;Bedon, Frank;Caron, Sebastien;Mansfield, Shawn D.;Levasseur, Caroline;Cooke, Janice E. K.;Blais, Sylvie;Tremblay, Laurence;Morency, Marie-Josee;Pavy, Nathalie;Grima-Pettenati, Jacqueline;Seguin, Armand;MacKay, John
通讯作者：
MacKay, John
针叶树R2R3-MYB转录因子：白云杉（Picea Glauca）的木制组织中的序列分析和基因表达。
影响因子：
5.3
作者：
Bedon, Frank;Grima-Pettenati, Jacqueline;Mackay, John
通讯作者：
Mackay, John
影响因子：
11.6
作者：
Bennett, Tom;van den Toorn, Albert;Scheres, Ben
通讯作者：
Scheres, Ben
影响因子：
10.5
作者：
Kubo, M;Udagawa, M;Demura, T
通讯作者：
Demura, T
影响因子：
11.6
作者：
Mitsuda, N;Seki, M;Ohme-Takagi, M
通讯作者：
Ohme-Takagi, M