In Vitro Fermentation of Selected Prebiotics and Their Effects on the Composition and Activity of the Adult Gut Microbiota.
In Vitro Fermentation of Selected Prebiotics and Their Effects on the Composition and Activity of the Adult Gut Microbiota.
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DOI：
10.3390/ijms19103097
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发表时间：
2018-10-10
影响因子：
5.6
通讯作者：
Raederstorff D
中科院分区：
文献类型：
--
作者：
Fehlbaum S;Prudence K;Kieboom J;Heerikhuisen M;van den Broek T;Schuren FHJ;Steinert RE;Raederstorff D
最近，益生元的概念已被重新审视，以扩展到不可消化的低聚糖之外，并且对选择性刺激的要求已扩展到包括双歧杆菌和乳酸菌以外的微生物群。在这里，研究了已知的和新的益生元对肠道微生物群的调节作用。在体外发酵筛选平台（i-screen）上接种成年粪便微生物群，暴露于不同浓度的膳食纤维（菊粉、α -连接半乳糖寡糖（α -GOS）、β -连接GOS、玉米芯和高纤维甘蔗的低聚木糖（XOS）和燕麦的β -葡聚糖），并将低聚果糖（FOS）阳性对照和阴性对照（不添加纤维）进行比较。所有膳食纤维都显示出益生元活性，与其他纤维相比，β -葡聚糖对微生物组成和代谢的影响更为明显。β -葡聚糖诱导普雷沃氏菌和玫瑰菌的生长，同时丙酸产量增加。菊粉和两种形式的GOS和XOS对微生物组成有很强的双歧致菌作用。当暴露于-葡聚糖和菊粉时，观察到丁酸盐的剂量反应效应。这项研究的结果支持α - gos、XOS和燕麦β -葡聚糖作为新型益生元的潜力，因为它们与微生物组组成和短链脂肪酸产生的积极变化有关，这表明它们对健康有潜在的好处。
Recently, the concept of prebiotics has been revisited to expand beyond non-digestible oligosaccharides, and the requirements for selective stimulation were extended to include microbial groups other than, and additional to, bifidobacteria and lactobacilli. Here, the gut microbiota-modulating effects of well-known and novel prebiotics were studied. An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition). All dietary fibers displayed prebiotic activity, with beta-glucan showing more distinct effects on the microbial composition and metabolism compared to the other fibers. Beta-glucan induced the growth of Prevotella and Roseburia with a concomitant increase in propionate production. Inulin and both forms of GOS and XOS had a strong bifidogenic effect on the microbial composition. A dose-response effect was observed for butyrate when exposed to beta-glucan and inulin. The findings of this study support the potential for alpha-GOS, XOS, and oat beta-glucan to serve as novel prebiotics, due to their association with the positive shifts in microbiome composition and short-chain fatty acid production that point to potential health benefits.
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影响因子：
64.8
作者：
Arumugam, Manimozhiyan;Raes, Jeroen;Pelletier, Eric;Le Paslier, Denis;Yamada, Takuji;Mende, Daniel R.;Fernandes, Gabriel R.;Tap, Julien;Bruls, Thomas;Batto, Jean-Michel;Bertalan, Marcelo;Borruel, Natalia;Casellas, Francesc;Fernandez, Leyden;Gautier, Laurent;Hansen, Torben;Hattori, Masahira;Hayashi, Tetsuya;Kleerebezem, Michiel;Kurokawa, Ken;Leclerc, Marion;Levenez, Florence;Manichanh, Chaysavanh;Nielsen, H. Bjorn;Nielsen, Trine;Pons, Nicolas;Poulain, Julie;Qin, Junjie;Sicheritz-Ponten, Thomas;Tims, Sebastian;Torrents, David;Ugarte, Edgardo;Zoetendal, Erwin G.;Wang, Jun;Guarner, Francisco;Pedersen, Oluf;de Vos, Willem M.;Brunak, Soren;Dore, Joel;Weissenbach, Jean;Ehrlich, S. Dusko;Bork, Peer
通讯作者：
Bork, Peer
影响因子：
15.5
作者：
Gorvitovskaia A;Holmes SP;Huse SM
通讯作者：
Huse SM
影响因子：
6.1
作者：
Dai, Zhuqing;Lyu, Wanyong;Zeng, Xiaoxiong
通讯作者：
Zeng, Xiaoxiong
影响因子：
4.2
作者：
Hughes, Simon A.;Shewry, Peter R.;Rastall, Robert A.
通讯作者：
Rastall, Robert A.
DOI：
10.1073/pnas.1000080107
发表时间：
2011-03-15
影响因子：
11.1
作者：
Caporaso, J. Gregory;Lauber, Christian L.;Knight, Rob
通讯作者：
Knight, Rob