Extraction and quantification of carbon nanotubes in biological matrices with application to rat lung tissue.
Extraction and quantification of carbon nanotubes in biological matrices with application to rat lung tissue.
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DOI：
10.1021/nn403302s
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发表时间：
2013-10-22
期刊：
影响因子：
17.1
通讯作者：
Westerhoff, Paul
中科院分区：
文献类型：
--
作者：
Doudrick, Kyle;Corson, Nancy;Oberdoerster, Guenter;Eder, Alison C.;Herckes, Pierre;Halden, Rolf U.;Westerhoff, Paul
从生物基质（如大鼠肺组织）中提取碳纳米管（CNTs）对于开发评估碳纳米管对环境和人类健康的暴露及毒性的量化方法是不可或缺的。评估了各种化学处理方法（包括可溶溶液（2.5%氢氧化钠/表面活性剂混合物）、氢氧化铵、硝酸、硫酸、盐酸、氢氟酸、过氧化氢和蛋白酶K）从大鼠肺组织中提取CNTs的能力。采用程序化热分析（PTA）对碳纳米管进行定量。两个碳纳米管分别代表碳纳米管热稳定性的下限（500°C）和上限（800°C）。所评估的八种化学试剂的回收率取决于(1)最小化CNTs氧化的能力，(2)从大鼠肺组织中去除干扰性背景碳的能力，以及(3)通过离心分离固相CNTs和液相溶解组织的能力。使用可溶性和蛋白酶K的两步提取方法被认为是最佳方法，使2.9±0.19µg CNT负载加到整个大鼠肺中的回收率为98±15%。由于其高收率和适用于纳米材料的低器官负荷，这种提取方法特别适合于体内研究，以量化清除率和肺和其他器官中保留的碳纳米管。
Extraction of carbon nanotubes (CNTs) from biological matrices such as rat lung tissue is integral to developing a quantification method for evaluating the environmental and human health exposure and toxicity of CNTs. The ability of various chemical treatment methods, including Solvable (2.5% sodium hydroxide/surfactant mixture), ammonium hydroxide, nitric acid, sulfuric acid, hydrochloric acid, hydrofluoric acid, hydrogen peroxide, and proteinase K, to extract CNTs from rat lung tissue was evaluated. CNTs were quantified using programmed thermal analysis (PTA). Two CNTs were used to represent the lower (500°C) and upper (800°C) PTA limit of CNT thermal stability. The recovery efficiency of each of the eight chemical reagents evaluated was found to depend on the ability to (1) minimize oxidation of CNTs, (2) remove interfering background carbon from the rat lung tissue, and (3) separate the solid-phase CNTs from the liquid-phase dissolved tissue via centrifugation. A two-step extraction method using Solvable and proteinase K emerged as the optimal approach, enabling a recovery of 98 ± 15% of a 2.9 ± 0.19 µg CNT loading that was spiked into whole rat lungs. Due to its high yield and applicability to low organ burdens of nanomaterials, this extraction method is particularly well suited for in vivo studies to quantify clearance rates and retained CNTs in lungs and other organs.
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影响因子：
10.4
作者：
Bonner JC;Silva RM;Taylor AJ;Brown JM;Hilderbrand SC;Castranova V;Porter D;Elder A;Oberdörster G;Harkema JR;Bramble LA;Kavanagh TJ;Botta D;Nel A;Pinkerton KE
通讯作者：
Pinkerton KE
影响因子：
8.6
作者：
Tchoul, Maxim N.;Ford, Warren T.;Arepalli, Sivaram
通讯作者：
Arepalli, Sivaram
影响因子：
--
作者：
Searl, A;Cullen, RT
通讯作者：
Cullen, RT
影响因子：
3.2
作者：
Singh, Dilip K.;Iyer, Parameswar K.;Giri, P. K.
通讯作者：
Giri, P. K.
影响因子：
2.5
作者：
Behler, K.;Osswald, S.;Gogotsi, Y.
通讯作者：
Gogotsi, Y.