Fine mapping of the root-knot nematode resistance gene Me1 in pepper (Capsicum annuum L.) and development of markers tightly linked to Me1
Fine mapping of the root-knot nematode resistance gene Me1 in pepper (Capsicum annuum L.) and development of markers tightly linked to Me1
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辣椒 (Capsicum annuum L.) 根结线虫抗性基因 Me1 的精细定位及与 Me1 紧密连锁的标记的开发
DOI：
10.1007/s11032-018-0793-2
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发表时间：
2018-03
影响因子：
3.1
通讯作者：
Wang Lihao
中科院分区：
文献类型：
--
作者：
Wang Xueying;Fazari Ariane;Cao Yacong;Zhang Zhenghai;Palloix Alain;Mao Shengli;Zhang Baoxi;Djian Caporalino Caroline;Wang Lihao
根结线虫（RKNs）在世界范围内严重危害包括辣椒在内的农作物。利用辣椒基因组中鉴定的抗性基因可能是控制RKNs的一种安全、经济的策略。在Me基因（Me1, Me3， Me7和N）中，Me1基因被定位到P9染色体上的一个簇上，Me1基因具有热稳定性和广谱抗性，这是毒性rkn难以克服的。在这项研究中，我们开发了几种密切相关的竞争性等位基因特异性PCR （KASPar）标记、简单序列重复（SSR）标记、序列特征扩增区（SCAR）标记和高分辨率融化（HRM）标记，用于定位rkn抗性基因。对948个个体（BC1和F2后代）的分析显示，Me1位于SCAR标记16880-1-V2和HRM标记16830-H-V2之间，与Me1分别发生了13次和0次重组事件。这些标记定位在一个132kb的区间，其中包括6个基因。开发与Me1密切相关的pcr标记将有助于辣椒品种抗RKN的标记辅助选择。在这些标记中，16830-H-V2和16830-CAPS存在于CA09g16830基因中，该基因被预测为推测的抗晚疫病蛋白同源基因R1A-3。该基因似乎是一个合适的Me1候选基因。
Root-knot nematodes (RKNs) can severely damage crops, including peppers, worldwide. The application of resistance genes identified in the Capsicum annuum genome may represent a safe and economically relevant strategy for controlling RKNs. Among the Me genes (Me1, Me3, Me7, and N) that have been mapped to a cluster on chromosome P9, Me1 confers a heat-stable and broad-spectrum resistance that is difficult for virulent RKNs to overcome. In this study, we developed several closely linked kompetitive allele-specific PCR (KASPar) markers, simple sequence repeat (SSR) markers, sequence characterized amplified region (SCAR) markers, and high-resolution melting (HRM) markers for the mapping of RKN-resistance genes. Analyses of 948 individuals (BC1 and F2 progenies) revealed that Me1 was located between SCAR marker 16880-1-V2 and HRM marker 16830-H-V2, with 13 and 0 recombination events with Me1, respectively. These markers were localized to a 132-kb interval, which included six genes. The development of several PCR-based markers closely linked to Me1 will be useful for the marker-assisted selection of RKN resistance in pepper cultivars. Among these markers, 16830-H-V2 and 16830-CAPS are present in the CA09g16830 gene, which is predicted to be a putative late blight resistance protein homolog R1A-3 gene. This gene appears to be a suitable Me1 candidate gene.
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影响因子：
4.4
作者：
Ashrafi H;Hill T;Stoffel K;Kozik A;Yao J;Chin-Wo SR;Van Deynze A
通讯作者：
Van Deynze A
影响因子：
7.2
作者：
van der Vossen, EAG;van der Voort, JNAMR;Klein-Lankhorst, RM
通讯作者：
Klein-Lankhorst, RM
影响因子：
3.2
作者：
A. Pegard;G. Brizzard;A. Fazari;O. Soucaze;P. Abad;C. Djian-Caporalino
通讯作者：
A. Pegard;G. Brizzard;A. Fazari;O. Soucaze;P. Abad;C. Djian-Caporalino
与 N 基因连锁的 SCAR 标记，可抵抗辣椒 (Capsicum annuum L.) 中的根结线虫 (Meloidogyne spp.)
影响因子：
4.3
作者：
Yun Xingfu;Mao Shengli;Hua Mingyan;Wnag Lihao;Zhang Zhenghai;Peng Deliang;Gu Xiaohui;Zhang Baoxi
通讯作者：
Zhang Baoxi
影响因子：
1.8
作者：
J. Thies;J. J. Ariss-J.
通讯作者：
J. Thies;J. J. Ariss-J.