A SCAR marker linked to the N gene for resistance to root knot nematodes (Meloidogyne spp.) in pepper (Capsicum annuum L.)
A SCAR marker linked to the N gene for resistance to root knot nematodes (Meloidogyne spp.) in pepper (Capsicum annuum L.)
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与 N 基因连锁的 SCAR 标记，可抵抗辣椒 (Capsicum annuum L.) 中的根结线虫 (Meloidogyne spp.)
DOI：
10.1016/j.scienta.2009.04.011
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发表时间：
2009-09
影响因子：
4.3
通讯作者：
Zhang Baoxi
中科院分区：
文献类型：
--
作者：
Yun Xingfu;Mao Shengli;Hua Mingyan;Wnag Lihao;Zhang Zhenghai;Peng Deliang;Gu Xiaohui;Zhang Baoxi
根结线虫（Meloidogyne spp.）是辣椒上重要的线虫害虫，在世界范围内引起严重的病害。目前还没有与线虫抗性N基因连锁的分子标记报道。以辣椒品种“卡罗莱纳奇迹”（Capsicum annuum L.）抗根结线虫的N基因甜椒新品系20080-5-29（C. annuum L.），以一个园艺性状优良的感根结线虫自交系及其F2代320株为材料。用南方根结线虫（Meloidogyne incognita）对亲本、F1代和F2代进行了抗感鉴定。采用“混合分离分析”方法对512对AFLP引物进行多态性分析。通过对AFLP标记在F2群体中的抗性、感病性和多态性分析，估算了该标记与N基因的遗传连锁距离。获得了一个AFLP标记E39/M41-339，并将其转移到一个SCAR标记上，扩增出与N抗性等位基因连锁的315 bp的DNA片段和与N+敏感等位基因连锁的331 bp的DNA片段。该标记与抗线虫N基因的距离为6.3cM。本研究为辣椒抗线虫性的分子标记辅助选择提供了一种有效的工具。
The root-knot nematodes (Meloidogyne spp.) are important nematode pests and cause serious diseases in pepper in the world. No molecular markers linked to the nematodes resistance N gene have been reported. In this paper, ‘Carolina Wonder’ (Capsicum annuum L.), a sweet pepper line resistant to root-knot nematode with N gene, ‘20080-5-29’ (C. annuum L.), an inbred line susceptible to root-knot nematode with good horticultural characteristics, and their F2progeny with 320 individuals were used as materials. Evaluation of resistance and susceptibility of parental lines, F1and F2progeny inoculated with root-knot nematodes (Meloidogyne incognita) were carried out. ‘Bulked segregant analysis’ method was used to search for polymorphic markers from 512 pairs of AFLP primers. Based on the assessment of resistance and susceptibility and polymorphism of the AFLP marker in F2population, the genetic linkage distance between the AFLP marker and the N gene was estimated. One AFLP marker E39/M41-339 was obtained and transferred to a SCAR marker amplifying a 315bp DNA fragment linked to the N resistant allele and a 331bp fragment linked to the N+susceptible allele. The distance between the molecular marker and the nematodes resistance N gene is 6.3cM. This research delivered a valuable tool for the marker assisted selection of nematodes resistance in pepper.
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DOI：
10.1051/agro:19850201
发表时间：
1985-01-01
期刊：
AGRONOMIE
影响因子：
--
作者：
HENDY, H;POCHARD, E;DALMASSO, A
通讯作者：
DALMASSO, A
影响因子：
2.7
作者：
CJ Sanguinetti;E. N. Dias;AJ Simpson
通讯作者：
CJ Sanguinetti;E. N. Dias;AJ Simpson
影响因子：
5.4
作者：
Djian-Caporalino, C.;Fazari, A.;Abad, P.
通讯作者：
Abad, P.
DOI：
10.21273/hortsci.32.5.937
发表时间：
1997-08
期刊：
--
影响因子：
--
作者：
J. Prince;Yiping Zhang;E. Radwanski;M. Kyle
通讯作者：
J. Prince;Yiping Zhang;E. Radwanski;M. Kyle
影响因子：
5.4
作者：
Djian-Caporalino, C;Pijarowski, L;Abad, P
通讯作者：
Abad, P