Material properties and strain distribution patterns of bovine growth plate cartilage vary with anatomic location and depth.
Material properties and strain distribution patterns of bovine growth plate cartilage vary with anatomic location and depth.
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DOI：
10.1016/j.jbiomech.2022.111013
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发表时间：
2022-03
影响因子：
2.4
通讯作者：
Ferguson, Virginia L.
中科院分区：
文献类型：
--
作者：
Fischenich, Kristine M.;Schneider, Stephanie E.;Neu, Corey P.;Payne, Karin A.;Ferguson, Virginia L.
本研究的目的是评估牛生长板软骨的本体材料特性和深度依赖性应变分布。我们假设，模量和应变分布是高度的深度，方向和位置依赖性。沿矢状面和冠状面对牛胫骨近端（1月龄）进行沿着切片，以创建约4 mm 2样本。数字图像相关（DIC）相结合的应力松弛试验的体积模量（切线和平衡）和深度相关的应变分布的评价。在Col-F染色以及组织学染色（番红-O/Fast绿色）后对样本的子集进行成像，以评价区域组织和基质组成。平均切线模量为4.25 ± 2.46 MPa，而平衡模量为0.86 ± 0.46 MPa。在方向（矢状面与冠状面）方面没有发现模量的显著差异，但关节内的矢状面位置是切线模量的显著预测因子。总体模量值从关节的外周到中线降低。由细胞密度和形状决定的深度依赖性细胞组织是高度可变的。这种异质性可能是一种生物增韧机制。峰值标准化应变最常见于肥大区。与静止区和增殖区相比，肥大区的模量显著较低。这项研究是第一次评估完整的生长板的模量和应变分布作为深度，方向和解剖位置的函数。未来的工作与生长板组织工程应考虑的位置和深度依赖性的天然组织的机械性能时，设计模拟结构。
The aim of this study was to assess the bulk material properties and depth-dependent strain distribution of bovine growth plate cartilage. We hypothesized that both moduli and strain distribution are highly depth-, orientation-, and location-dependent. Bovine proximal tibias (1-month-old) were sliced along the sagittal and coronal planes to create ~4 mm2 samples. Digital image correlation (DIC) was combined with stress relaxation tests for evaluation of bulk modulus (tangent and equilibrium) and depth-dependent strain distribution. A subset of samples was imaged after Col-F staining as well as histological staining (Safranin-O/Fast Green) to evaluate zonal organization and matrix composition. The mean tangent modulus was 4.25 ± 2.46 MPa while the equilibrium modulus was 0.86 ± 0.46 MPa. No significant differences in moduli were found with respect to orientation (sagittal vs coronal face), but sagittal location within the joint was a significant predictor for tangent modulus. Overall moduli values decreased from the periphery to the midline of the joint. Depth-dependent cellular organization, determined by cell density and shape, was highly variable. This heterogeneity may be a biological toughening mechanism. Peak normalized strains were observed most often in the hypertrophic zone. Modulus was significantly lower in the hypertrophic zone as compared to the resting and proliferative zones. This study is the first to evaluate moduli and strain distribution in intact growth plates as a function of depth, orientation, and anatomic location. Future work with growth plate tissue engineering should consider the location- and depth-dependent nature of the native tissue mechanical properties when designing mimetic constructs.
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影响因子：
2.8
作者：
COHEN, B;CHORNEY, GS;MOW, VC
通讯作者：
MOW, VC
影响因子：
2.8
作者：
COHEN, B;CHORNEY, GS;MOW, VC
通讯作者：
MOW, VC
影响因子：
2.8
作者：
Schinagl, RM;Gurskis, D;Sah, RL
通讯作者：
Sah, RL
DOI：
10.1302/0301-620x.71b1.2914983
发表时间：
1989-01-01
影响因子：
--
作者：
BROUGHTON, NS;DICKENS, DRV;MENELAUS, MB
通讯作者：
MENELAUS, MB
影响因子：
2.4
作者：
Klein, Travis J.;Chaudhry, Manu;Sah, Robert L.
通讯作者：
Sah, Robert L.