The predicted secretome of the plant pathogenic fungus Fusarium graminearum: a refined comparative analysis.
The predicted secretome of the plant pathogenic fungus Fusarium graminearum: a refined comparative analysis.
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DOI：
10.1371/journal.pone.0033731
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Hammond-Kosack KE
中科院分区：
文献类型：
--
作者：
Brown NA;Antoniw J;Hammond-Kosack KE
禾谷镰刀菌在侵染开花期的花组织时，与寄主小麦形成了密切的联系。在延长的感染潜伏期内，活体病原菌和宿主细胞之间必须进行细胞外通讯，这意味着分泌的真菌蛋白发挥了作用。与真菌菌丝接触的小麦细胞随后死亡，细胞内菌丝定植导致可见的疾病症状的发展。自从2007年利用TargetP预测分泌组的原始基因组注释分析完成以来，经过BREAD和MIPS研究所的共同努力，禾谷镰刀菌的基因调用发生了很大的变化。由于对基因组的修改和最近的发现表明分泌蛋白在毒力中起到了作用，禾谷镰刀菌的分泌组被重新研究。在目前的研究中，通过结合几种生物信息学方法预测了一个精制的禾谷镰刀菌分泌组。这一策略增加了识别真正分泌的蛋白质的可能性。预测了一个由574个蛋白质组成的分泌组，其中99%的蛋白质被转录证据支持。对已注释和未注释的分泌蛋白的功能进行了探讨。讨论了注释蛋白的潜在作用(S)，包括可能的酶、植物毒素和抗真菌药物。未注释蛋白质的特征包括分析Pfam结构域和与已知真菌效应器相关的特征，例如，小尺寸、富含半胱氨酸和包含内部氨基酸重复的特征。对57个真菌和卵菌基因组的综合比较基因组分析表明，预测的禾谷镰刀菌分泌蛋白中只有一小部分可以被认为是物种或测序菌株特异性的。
The fungus Fusarium graminearum forms an intimate association with the host species wheat whilst infecting the floral tissues at anthesis. During the prolonged latent period of infection, extracellular communication between live pathogen and host cells must occur, implying a role for secreted fungal proteins. The wheat cells in contact with fungal hyphae subsequently die and intracellular hyphal colonisation results in the development of visible disease symptoms. Since the original genome annotation analysis was done in 2007, which predicted the secretome using TargetP, the F. graminearum gene call has changed considerably through the combined efforts of the BROAD and MIPS institutes. As a result of the modifications to the genome and the recent findings that suggested a role for secreted proteins in virulence, the F. graminearum secretome was revisited. In the current study, a refined F. graminearum secretome was predicted by combining several bioinformatic approaches. This strategy increased the probability of identifying truly secreted proteins. A secretome of 574 proteins was predicted of which 99% was supported by transcriptional evidence. The function of the annotated and unannotated secreted proteins was explored. The potential role(s) of the annotated proteins including, putative enzymes, phytotoxins and antifungals are discussed. Characterisation of the unannotated proteins included the analysis of Pfam domains and features associated with known fungal effectors, for example, small size, cysteine-rich and containing internal amino acid repeats. A comprehensive comparative genomic analysis involving 57 fungal and oomycete genomes revealed that only a small number of the predicted F. graminearum secreted proteins can be considered to be either species or sequenced strain specific.
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