The Complete Chloroplast Genome Sequence of Machilus chuanchienensis (Lauraceae): Genome Structure and Phylogenetic Analysis.

The Complete Chloroplast Genome Sequence of Machilus chuanchienensis (Lauraceae): Genome Structure and Phylogenetic Analysis.
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川池润楠(樟科)叶绿体全基因组序列:基因组结构和系统发育分析

DOI:
10.3390/genes13122402
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发表时间:
2022-12-18
期刊:
影响因子:
3.5
通讯作者:
--
中科院分区:
生物学3区
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--
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川黔润楠是我国西南地区的一种生态树种。老鹰茶是中国茶文化中历史悠久的民族传统茶饮料,用老鹰叶制作老鹰茶具有重要的价值。全叶绿体基因组结构简单、高度保守,是樟科植物系统发育研究的理想模型。樟科植物cp基因组全序列的报道很多,但樟科植物M.川剑草在此,使用下一代测序(NGS)对M.川坚cp基因组然后,进行了全面的比较基因组分析。结果表明,M.川穹cp基因组全长152,748个碱基对(bp),GC含量为39.15%,编码126个注释基因,其中包括8个核糖体RNA(rRNA)、36个转运蛋白RNA(tRNA)和82个蛋白质编码基因。cp基因组呈现典型的四分结构,由大的单拷贝(LSC; 93,811)区、小的单拷贝(SSC; 18,803)区和反向重复序列(IRs; 20,067)区组成,共包含92个SSR位点。系统发育关系分析表明,M. chuanchienensis是M. balansae,M. melanophylla和M.小花对这一关键物种的进一步研究可能会从这些发现中受益匪浅。
Machilus chuanchienensis is an ecological tree distributed in southwestern China. It has a significant valuation with making Hawk tea using its leaves, an ethnic traditional tea-like beverage with a long history in Chinese tea culture. The whole chloroplast (cp) genome is an ideal model for the phylogenetic study of Lauraceae because of its simple structure and highly conserved features. There have been numerous reports of complete cp genome sequences in Lauraceae, but little is known about M. chuanchienensis. Here, the next-generation sequencing (NGS) was used to sequence the M. chuanchienensis cp genome. Then, a comprehensive comparative genome analysis was performed. The results revealed that the M. chuanchienensis’s cp genome measured 152,748 base pairs (bp) with a GC content of 39.15% and coded 126 genes annotated, including comprising eight ribosomal RNA (rRNA), 36 transporter RNA (tRNA), and 82 protein-coding genes. In addition, the cp genome presented a typical quadripartite structure comprising a large single-copy (LSC; 93,811) region, a small single-copy (SSC; 18,803) region, and the inverted repeats (IRs; 20,067) region and contained 92 simple sequence repeat (SSR) locus in total. Phylogenetic relationships of 37 species indicated that M. chuanchienensis was a sister to M. balansae, M. melanophylla, and M. minutiflora. Further research on this crucial species may benefit significantly from these findings.
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