Molecular epidemiology of Streptococcus pneumoniae isolated from pediatric community-acquired pneumonia in pre-conjugate vaccine era in Western China.
Molecular epidemiology of Streptococcus pneumoniae isolated from pediatric community-acquired pneumonia in pre-conjugate vaccine era in Western China.
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DOI：
10.1186/s12941-020-00410-x
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发表时间：
2021-01-06
影响因子：
5.7
通讯作者：
McGrath E
中科院分区：
文献类型：
--
作者：
Liang Z;Fu J;Li L;Yi R;Xu S;Chen J;Ye X;McGrath E
肺炎链球菌（S.pneumoniae）是最常见的病原体之一，可导致全世界儿科感染的发病率和死亡率。本研究旨在描述从中国西部儿童中发现的引起社区获得性肺炎（CAP）的肺炎链球菌的表型和分子特征。我们回顾性招募了 5 岁以下被诊断患有 CAP 的儿科患者。对所有 419 株肺炎链球菌分离株进行了抗生素敏感性、血清型、毒力基因、抗性基因和序列类型测试。通过对应分析测试分子特征之间的潜在关系。大多数肺炎链球菌对红霉素、四环素、克林霉素和甲氧苄啶-磺胺甲恶唑耐药，其中93.8%的分离株具有多重耐药性。主要 ST 为 ST271 (30.8%) 和 ST320 (12.2%)，主要血清型为 19F (46.8%)、6B (11.5%)、23F (9.5%) 和 19A (9.3%)。 PCV-7和PCV-13的覆盖率分别为73.03%和86.16%，而<1岁和1-2岁儿童PCV13的覆盖率较高，分别为93.18%和93.62%。我们还观察到，CC271 比非 CC271 分离株表达更多的 mef (A/E)、lytA、rlrA 和 sipA。此外，分子特征之间存在很强的对应关系。 PCV13的高覆盖率表明在中国西部地区引入PCV13疫苗的必要性。我们的研究结果强调了监测多个分子特征的价值，为开发未来的肺炎球菌疫苗提供新的指导。
Streptococcus pneumoniae (S. pneumoniae) is one of the most common pathogens which can cause morbidity and mortality in pediatric infections worldwide. This study aimed to describe the phenotypic and molecular characteristics of community-acquired pneumonia (CAP)-causing S. pneumoniae recovered from children in Western China. We retrospectively enrolled pediatric patients younger than 5 years diagnosed with CAP. All 419 S. pneumoniae isolates were tested for antibiotic susceptibility, serotypes, virulence genes, resistance genes, and sequence types. The potential relationships between molecular characteristics were tested by correspondence analysis. Most of S. pneumoniae isolates were resistant to erythromycin, tetracycline, clindamycin and trimethoprim-sulfamethoxazole, with 93.8% isolates classified as multidrug resistant. The dominant STs were ST271 (30.8%) and ST320 (12.2%), while the prevailing serotypes were 19F (46.8%), 6B (11.5%), 23F (9.5%) and 19A (9.3%). The coverage rates of PCV-7 and PCV-13 were 73.03% and 86.16%, while the coverage rates of PCV13 among children aged < 1 year and 1–2 years were high in 93.18% and 93.62%. We also observed that CC271 expressed more of mef (A/E), lytA, rlrA and sipA than non-CC271 isolates. Moreover, there were strong corresponding relationships between molecular characteristics. The high coverage rate of PCV13 suggests the necessity of introducing the PCV13 vaccine in Western China. Our findings underscore the value of monitoring multiple molecular characteristics to provide new guidance for developing future pneumococcal vaccines.
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影响因子：
4.2
作者：
Bowers JR;Driebe EM;Nibecker JL;Wojack BR;Sarovich DS;Wong AH;Brzoska PM;Hubert N;Knadler A;Watson LM;Wagner DM;Furtado MR;Saubolle M;Engelthaler DM;Keim PS
通讯作者：
Keim PS
影响因子：
5.2
作者：
Hjalmarsdottir, M. A.;Kristinsson, K. G.
通讯作者：
Kristinsson, K. G.
影响因子：
4.2
作者：
Li, Q. H.;Yao, K. H.;Yang, Y. H.
通讯作者：
Yang, Y. H.
影响因子：
14.2
作者：
Hsieh, Y. -C.;Huang, Y. -C.;Hsueh, P. -R.
通讯作者：
Hsueh, P. -R.
DOI：
10.1099/00221287-144-11-3049
发表时间：
1998-11-01
期刊：
MICROBIOLOGY-UK
影响因子：
--
作者：
Enright, MC;Spratt, BG
通讯作者：
Spratt, BG