Accumulation of secretory vesicles in the lacrimal gland epithelia is related to non-Sjögren's type dry eye in visual display terminal users.
Accumulation of secretory vesicles in the lacrimal gland epithelia is related to non-Sjögren's type dry eye in visual display terminal users.
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DOI：
10.1371/journal.pone.0043688
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Tsubota K
中科院分区：
文献类型：
--
作者：
Kamoi M;Ogawa Y;Nakamura S;Dogru M;Nagai T;Obata H;Ito M;Kaido M;Kawakita T;Okada Y;Kawakami Y;Shimmura S;Tsubota K
先前在视觉显示终端（VDT）使用者中观察到的非干燥综合征（非SS）型干眼大鼠模型中的观察结果表明，泪腺上皮细胞中的分泌囊泡（SV）积聚促成了该病症。在这里，研究这种可能性在人类中，我们比较了泪腺组织学和腺泡上皮细胞的细胞质中的SV面积百分比，使用光学显微镜和透射电子显微镜，在VDT工作相关的非SS干眼症（VDT组），SS诱导的干眼症，和尸检正常对照组的患者。此外，在泪腺组织切片中对VAMP 8（囊泡相关膜蛋白8，一种外分泌途径分子）和Rab3D（成熟囊泡标记物）进行组织化学检查。VDT组泪腺腺泡较SS组大，SV面积百分比显著高于正常对照组（P = 0.021）和SS组（P = 0.004）。 免疫组化显示VDT组和SS组VAMP8的异常分布。Rab3D在VDT组腺泡上皮细胞胞浆中的表达明显高于正常对照组。视屏显示终端组使用视屏显示终端的时间明显长于其他组。这些结果表明，过多的SV积累在腺泡上皮细胞可能有助于减少泪液分泌的VDT用户。
Previous observations in a rat model of a non-Sjögren's syndrome (non-SS) type of dry eye seen in users of visual display terminals (VDT) indicated that secretory vesicle (SV) accumulation in the lacrimal gland epithelia contributes to the condition. Here, to examine this possibility in humans, we compared the lacrimal gland histology and percent SV area in the cytoplasm of acinar epithelial cells using light microscopy and transmission electron microscopy, in patients with VDT work-related non-SS dry-eye (VDT group), SS-induced dry-eye, and autopsied normal controls. In addition, the VAMP8 (vesicle-associated membrane protein 8, an exocrine-pathway molecule) and Rab3D (mature vesicle marker) were histochemically examined in lacrimal gland tissue sections. The lacrimal gland acini were larger in the VDT group than in the SS group, and the percent SV area was significantly higher in the VDT group than in the normal controls (P = 0.021) or SS group (P = 0.004). Immunostaining revealed abnormal distributions of VAMP8 in the VDT and SS groups. Rab3D was more strongly expressed in the cytoplasm of acinar epithelial cells in the VDT group than in that of normal controls. The duration of VDT use was significantly longer in the VDT group than in the other groups. These findings suggest that excessive SV accumulation in the acinar epithelia may contribute to the reduced tear secretion in VDT users.
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影响因子：
--
作者：
FOX, RI;ROBINSON, CA;HOWELL, FV
通讯作者：
HOWELL, FV
影响因子：
4.4
作者：
Nakamura, S;Shibuya, M;Tsubota, K
通讯作者：
Tsubota, K
影响因子：
6.4
作者：
Rocha, Eduardo M.;Alves, Monica;Rios, J. David;Dartt, Darlene A.
通讯作者：
Dartt, Darlene A.
影响因子：
3.4
作者：
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通讯作者：
Belmonte, C
影响因子：
2.2
作者：
Kokolakis, Georgios;Panagis, Lambros;Kruger-Krasagakis, Sabine
通讯作者：
Kruger-Krasagakis, Sabine