Identification of novel Z/W chromosome-specific markers from the giant freshwater prawn Macrobrachium rosenbergii

Identification of novel Z/W chromosome-specific markers from the giant freshwater prawn Macrobrachium rosenbergii
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罗氏沼虾新型 Z/W 染色体特异性标记的鉴定

DOI:
10.1016/j.aaf.2022.01.003
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发表时间:
2022-01
影响因子:
--
通讯作者:
Qiu Gaofeng
Qiu Gaofeng
中科院分区:
--
文献类型:
--
作者:
Liu Xue;Zhou Lingxia;Luo Biyun;Qian Hongli;Ye Baoqing;Ma Keyi;Qiu Gaofeng

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罗氏沼虾(Macrobrachium rosenbergium)是我国重要的经济养殖品种。性染色体特异性标记是性别鉴定和性染色体区分的关键。本文根据M. Rosenbertman我们发现了稳定的性连锁标记进一步的核苷酸比对分析显示,扩增的W染色体和Z染色体片段之间的核苷酸相似性为83.42%。qPCR证实,Z染色体特异性片段在男性中的出现频率约为女性的两倍。同样,W染色体特异性片段存在于超级女性约两倍,往往与女性相比。我们的研究结果阐明了ZW/ZZ性别决定类型,并有助于探索性染色体上存在的性别相关或性别决定基因。罗森伯托。
The giant freshwater prawn (Macrobrachium rosenbergii) is a commercially cultured species in China. A sex chromosome-specific marker is crucial for sex identification and distinguishing sex chromosomes. Here, based on the results of reduced-representation genome sequencing and genome survey ofM. rosenbergii, we identified steady sex-linked markers. Further nucleotide alignment analysis revealed 83.42% nucleotide similarity between the amplified W- and Z-chromosome fragments. qPCR confirmed that Z chromosome-specific fragments were present in males approximately twice as often compared to females. Similarly, the W chromosome-specific fragments were present in super females approximately twice as often compared to females. Our findings elucidate the ZW/ZZ sex determination type and contribute to exploring sex-related or sex-determining genes present on the sex chromosomes ofM. rosenbergii.
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发表时间: 2006-06-15
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DOI: --
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