Comparative Genomic Analysis of Streptococcus dysgalactiae subspecies dysgalactiae Isolated From Bovine Mastitis in China.
Comparative Genomic Analysis of Streptococcus dysgalactiae subspecies dysgalactiae Isolated From Bovine Mastitis in China.
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中国牛乳腺炎停乳链球菌停乳亚种的比较基因组分析
DOI：
10.3389/fmicb.2021.751863
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发表时间：
2021
影响因子：
5.2
通讯作者：
Han B
中科院分区：
文献类型：
--
作者：
Xu S;Liu Y;Gao J;Zhou M;Yang J;He F;Kastelic JP;Deng Z;Han B
停乳链球菌停乳症（SDSD）是世界范围内引起牛乳腺炎的最普遍的病原体之一。然而，有一个缺乏全面的信息，遗传多样性，完整的档案，毒力因子（VF）和抗生素耐药（AMR）基因的SDSD与牛乳房炎在中国。在这项研究中，从2016年11月至2019年6月，从中国7个省的17个牛群中共采集了674份牛奶样本，包括509例临床乳腺炎和165例亚临床乳腺炎病例的样本。所有SDSD分离株均纳入基于16 S rRNA和多位点序列分型（MLST）的系统发育分析。此外，对12个代表性SDSD分离株进行全基因组测序，以筛选VF和AMR基因，并确定泛基因组、核心基因组和辅助基因组。乳房炎乳样中SDSD的患病率为7.57%（51/674）。基于16 SrRNA的系统发育分析将51株SDSD分离株分为4个聚类，基于MLST的系统发育分析将51株SDSD分离株分为11个序列型，其中包括6个已登记的ST和5个新的ST（ST 521、ST 523、ST 526、ST 527、ST 529），分属2个不同的克隆复合体（CC）和4个单基因。根据WGS信息，对12个分离株的108个VF基因进行了11类鉴定。此外，在11个类别中鉴定了23个AMR基因。全基因组、核心基因组和辅助基因组分别由2，663、1，633和699个基因组成。这些结果提供了一个全面的谱SDSD毒力和抗性基因以及系统发育关系，在中国北方乳房炎相关SDSD。
Streptococcus dysgalactiae subsp. dysgalactiae (SDSD) is one of the most prevalent pathogens causing bovine mastitis worldwide. However, there is a lack of comprehensive information regarding genetic diversity, complete profiles of virulence factors (VFs), and antimicrobial resistance (AMR) genes for SDSD associated with bovine mastitis in China. In this study, a total of 674 milk samples, including samples from 509 clinical and 165 subclinical mastitis cases, were collected from 17 herds in 7 provinces in China from November 2016 to June 2019. All SDSD isolates were included in phylogenetic analysis based on 16S rRNA and multi-locus sequence typing (MLST). In addition, whole genome sequencing was performed on 12 representative SDSD isolates to screen for VFs and AMR genes and to define pan-, core and accessory genomes. The prevalence of SDSD from mastitis milk samples was 7.57% (51/674). According to phylogenetic analysis based on 16S rRNA, 51 SDSD isolates were divided into 4 clusters, whereas based on MLST, 51 SDSD isolates were identified as 11 sequence types, including 6 registered STs and 5 novel STs (ST521, ST523, ST526, ST527, ST529) that belonged to 2 distinct clonal complexes (CCs) and 4 singletons. Based on WGS information, 108 VFs genes in 12 isolates were determined in 11 categories. In addition, 23 AMR genes were identified in 11 categories. Pan-, core and accessory genomes were composed of 2,663, 1,633 and 699 genes, respectively. These results provided a comprehensive profiles of SDSD virulence and resistance genes as well as phylogenetic relationships among mastitis associated SDSD in North China.
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影响因子：
4.1
作者：
Alves-Barroco, Cinthia;Roma-Rodrigues, Catarina;Santos-Sanches, Ilda
通讯作者：
Santos-Sanches, Ilda
影响因子：
2.2
作者：
Fernandez, G.;Barreal, M. L.;Gonzalez-Palencia, J.
通讯作者：
Gonzalez-Palencia, J.
影响因子：
3.5
作者：
Godden, S. M.;Royster, E.;Green, H.
通讯作者：
Green, H.
影响因子：
7.8
作者：
Diaz-Dinamarca, Diego A.;Hernandez, Carlos;Vasquez, Abel E.
通讯作者：
Vasquez, Abel E.
关于停乳链球菌亚种的新见解。停乳分离株。
影响因子：
5.2
作者：
Alves-Barroco C;Caço J;Roma-Rodrigues C;Fernandes AR;Bexiga R;Oliveira M;Chambel L;Tenreiro R;Mato R;Santos-Sanches I
通讯作者：
Santos-Sanches I