New Insights on Streptococcus dysgalactiae subsp. dysgalactiae Isolates.
New Insights on Streptococcus dysgalactiae subsp. dysgalactiae Isolates.
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关于停乳链球菌亚种的新见解。停乳分离株。
DOI：
10.3389/fmicb.2021.686413
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发表时间：
2021
影响因子：
5.2
通讯作者：
Santos-Sanches I
中科院分区：
文献类型：
--
作者：
Alves-Barroco C;Caço J;Roma-Rodrigues C;Fernandes AR;Bexiga R;Oliveira M;Chambel L;Tenreiro R;Mato R;Santos-Sanches I
停乳链球菌停乳症（SDSD）一直被认为是严格的动物病原体。然而，最近的人类感染报告表明，该亚种的生态位扩张，这可能是毒力基因获得增加其致病性的结果。先前的研究报道了牛SDSD中存在化脓性链球菌的毒力基因（2002-2003年采集）;然而，这些移动的遗传元件的身份仍有待澄清。因此，本研究旨在表征2011-2013年收集的SDSD分离株，并将其与2002-2003年收集的SDSD分离株和国家生物技术信息中心（NCBI）数据库中的化脓性链球菌基因组进行比较，包括人SDSD和S.停乳亚种equisimilis（SDSE）菌株来追踪牛SDSD基因型的时间变化。从管家基因的分析中可以看出，人SDSD和SDSE之间的遗传关系非常密切，而牛SDSD分离株似乎更趋不同。结果表明，所有牛SDSD均具有规则间隔短回文重复序列（CRISPR）/Cas IIA系统。该系统在牛SDSD分离株中的广泛存在、重复序列的高度保守性以及在间隔区中观察到的多态性可以被认为是该系统活性的指标。总体而言，比较分析表明，牛SDSD分离株携带S. speK、speC、speL、speM、spd 1和sdn毒力基因。化脓性前噬菌体。我们的数据表明，这些基因保持随着时间的推移，似乎是唯一的牛SDSD菌株的属性。虽然本研究中表征的牛SDSD基因组没有测序，但数据集，包括牛SDSD和S.化脓菌菌株，可能表明水平遗传转移事件发生在生境分离之前。除佐贺基因外，所有牛SDSD分离株对编码链球菌溶血素S的操纵子基因均为阴性，而在所有SDSE和人SDSD菌株中均检测到该操纵子的存在。本研究的数据集表明，应该重新考虑亚种“停乳”和“equisimilis”之间的分离。然而，一项研究，包括最全面的收集菌株从不同的环境将需要明确的结论，关于这两个类群。
Streptococcus dysgalactiae subsp. dysgalactiae (SDSD) has been considered a strict animal pathogen. Nevertheless, the recent reports of human infections suggest a niche expansion for this subspecies, which may be a consequence of the virulence gene acquisition that increases its pathogenicity. Previous studies reported the presence of virulence genes of Streptococcus pyogenes phages among bovine SDSD (collected in 2002–2003); however, the identity of these mobile genetic elements remains to be clarified. Thus, this study aimed to characterize the SDSD isolates collected in 2011–2013 and compare them with SDSD isolates collected in 2002–2003 and pyogenic streptococcus genomes available at the National Center for Biotechnology Information (NCBI) database, including human SDSD and S. dysgalactiae subsp. equisimilis (SDSE) strains to track temporal shifts on bovine SDSD genotypes. The very close genetic relationships between humans SDSD and SDSE were evident from the analysis of housekeeping genes, while bovine SDSD isolates seem more divergent. The results showed that all bovine SDSD harbor Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas IIA system. The widespread presence of this system among bovine SDSD isolates, high conservation of repeat sequences, and the polymorphism observed in spacer can be considered indicators of the system activity. Overall, comparative analysis shows that bovine SDSD isolates carry speK, speC, speL, speM, spd1, and sdn virulence genes of S. pyogenes prophages. Our data suggest that these genes are maintained over time and seem to be exclusively a property of bovine SDSD strains. Although the bovine SDSD genomes characterized in the present study were not sequenced, the data set, including the high homology of superantigens (SAgs) genes between bovine SDSD and S. pyogenes strains, may indicate that events of horizontal genetic transfer occurred before habitat separation. All bovine SDSD isolates were negative for genes of operon encoding streptolysin S, except for sagA gene, while the presence of this operon was detected in all SDSE and human SDSD strains. The data set of this study suggests that the separation between the subspecies “dysgalactiae” and “equisimilis” should be reconsidered. However, a study including the most comprehensive collection of strains from different environments would be required for definitive conclusions regarding the two taxa.
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影响因子：
14.9
作者：
Grissa, Ibtissem;Vergnaud, Gilles;Pourcel, Christine
通讯作者：
Pourcel, Christine
影响因子：
10.7
作者：
Abdelsalam, M.;Fujino, M.;Warda, M.
通讯作者：
Warda, M.
影响因子：
4.1
作者：
Alves-Barroco, Cinthia;Roma-Rodrigues, Catarina;Santos-Sanches, Ilda
通讯作者：
Santos-Sanches, Ilda
影响因子：
2.8
作者：
Hu, Tong;Cui, Yanhua;Qu, Xiaojun
通讯作者：
Qu, Xiaojun
影响因子：
4.9
作者：
Brenciani, Andrea;Bacciaglia, Alessandro;Giovanettil, Eleonora
通讯作者：
Giovanettil, Eleonora